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R6504

Sigma-Aldrich

Milieu RPMI-1640

With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonyme(s) :

Roswell Park Memorial Institute 1640 medium

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About This Item

Numéro MDL:
MFCD00217820
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Niveau de qualité

500

Forme

powder

Technique(s)

cell culture | mammalian: suitable

Composants

L-glutamine: yes
sodium pyruvate: no
HEPES: no
NaHCO3: no
phenol red: yes

Conditions d'expédition

ambient

Température de stockage

2-8°C

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Description générale

Le milieu RPMI 1640 a été mis au point par Moore et ses collaborateurs, au Roswell Park Memorial Institute (RPMI), en 1966. Version modifiée du milieu 5A de McCoy, il a été formulé pour permettre la culture de cellules lymphoblastoïdes en suspension, mais on sait depuis qu'il convient aussi à une grande diversité de cellules adhérentes. Initialement conçu pour être utilisé avec un supplément à base de sérum, le milieu RPMI 1640 s'est révélé adapté à la culture sans sérum de différentes lignées cellulaires. Il est aussi couramment employé dans les protocoles de fusion ainsi que pour la croissance de cellules hybrides. Ce milieu convient à la culture de leucocytes normaux et néoplasiques humains.
Roswell Park Memorial Institute medium is commonly known as RPMI. It is a form of medium used in cell culture and tissue culture. This medium contains phosphate and is formulated for use in a 5% carbon dioxide atmosphere. It is used for the growth of human lymphoid cells.

Application

RPMI-1640 Medium is used for the following applications:
  • used in cell culture medium (i.e. Murine thymoma EL-4 cells were cultured in RPMI 1640 medium supplemented with other components)[1]
  • used for the mycobacterial infection of splenocytes (in antibiotic-free RPMI 1640 medium)[2]
  • used for cell isolation and culture[3][4]
  • used in culture medium (as one of the component) during lymphocyte separation and culture, ELISPOT Assay, Ex Vivo proliferation assay[5]
  • used in medium for gp39 gene expression and CD40-Immunoglobuin binding assays[6]
  • used for the preparation of antifungal agents[7][8]

Quantité

Formulated to contain 10.4 grams of powder per liter of medium.

Reconstitution

Supplement with 2.0 g/L sodium bicarbonate.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
0000416044
0000416044
0000415982
0000415982
0000382657

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Hui Tian et al.
Analytical chemistry, 88(23), 11384-11389 (2016-11-02)
MicroRNA (miRNA) analysis in a single cell is extremely important because it allows deep understanding of the exact correlation between the miRNAs and cell functions. Herein, we wish to report a highly sensitive and precisely quantitative assay for miRNA detection
Manuel Cuenca-Estrella et al.
Antimicrobial agents and chemotherapy, 46(11), 3644-3647 (2002-10-18)
A two-laboratory study was performed to evaluate the correlation between the NCCLS M27-A and EUCAST microdilution procedures for antifungal testing of Candida spp. A panel of 109 bloodstream isolates was tested against amphotericin B, flucytosine, fluconazole, and itraconazole. Overall, the
M E Klepser et al.
Antimicrobial agents and chemotherapy, 41(6), 1392-1395 (1997-06-01)
Time-kill curves were determined for three isolates of Candida albicans tested against fluconazole and amphotericin B at multiples of the MIC. Fluconazole produced fungistatic activity, with concentration-related growth effects observed over a narrow range of concentrations. Amphotericin B exhibited fungicidal
M Farrington et al.
Proceedings of the National Academy of Sciences of the United States of America, 91(3), 1099-1103 (1994-02-01)
Common variable immunodeficiency (CVI) is characterized by hypogammaglobulinemia and recurrent bacterial infections due to failure of CVI B cells to differentiate in vivo into immunoglobulin-secreting plasma cells. We hypothesized that T-cell dysfunction resulting in abnormal contact-mediated B-cell activation may play
Mathias Lichterfeld et al.
The Journal of experimental medicine, 200(6), 701-712 (2004-09-24)
Virus-specific CD8(+) T cells are associated with declining viremia in acute human immunodeficiency virus (HIV)1 infection, but do not correlate with control of viremia in chronic infection, suggesting a progressive functional defect not measured by interferon gamma assays presently used.
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Questions

1–4 of 4 Questions  

  1. how much media is added to prepare 10ml rpmi media?

    1 answer

    1. This product is a powder form of RPMI. This formulation requires 10.4 grams to prepare one liter of medium. The addition of 2.0 g/L sodium bicarbonate is also required. To prepare 10 ml of liquid medium from this item would require 104 mg of the powder product plus the addition of 20 mg of sodium bicarbonate. Note that R8758, the prepared liquid medium of this version of RPMI, may be of interest.

      Please see the link below to compare the powder and liquid medium formulations:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/209/459/r6504for.pdf

      Helpful?

  2. Why doesn't the powder dissolve in deionised water for 3 days?

    1 answer

    1. The product issue you are reporting should be investigated by our Technical Service team who can assist you with further troubleshooting and/or investigation. We kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice, and click on "Report Product Issues" under the Products Section with all the required information so that a member of our team can reach out to you to assist further. Thank you.

      Helpful?

  3. Buongiorno Gentilissima/o, volevo chiedervi maggiori informazioni per quanto riguarda la differenza tra il terreno R6504-10L e il R4130-10L: sono entrambi in polvere? da cosa deriva la differenza di prezzo? funziona per la crescita di muffe? Grazie

    1 answer

    1. The only difference between these two formulations is the addition of HEPES buffer to the R4103. All other components are identical. None of the classical mammalian culture media are tested for culture or expansion of molds. However, sterile solutions of this media, if handled improperly, have been known to become easily and heavily contaminated the airborne contaminants such as mold spores. To compare these media formulations please see the link below to access the RPMI-1640 formulations table:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/209/459/r6504for.pdf

      Helpful?

  4. is this product available in powder form

    1 answer

    1. Product R6504 is a powdered formulation of RPMI. It is formulated to contain 10.4 grams of powder per liter of medium and requires supplement with 2.0 g/L sodium bicarbonate. Its 1X liquid product equivalent is Product R8758 (https://www.sigmaaldrich.com/US/en/product/sigma/r8758

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