R1003

Ribonuclease T₁ from Aspergillus oryzae

ammonium sulfate suspension, 300,000-600,000 units/mg protein

• Synonyme(s) : Guanyloribonuclease, Ribonucleate 3′-guanylo-oligonucleotidohydrolase
• Numéro CAS: 9026-12-4
• Numéro de classification (Commission des enzymes): 3.1.27.3 (BRENDA, IUBMB)
• Numéro CE : 232-809-1
• Numéro MDL: MFCD00132191
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: Aspergillus sp. (Aspergillus oryzae)
• Niveau de qualité: 200
• Forme: ammonium sulfate suspension
• Activité spécifique: 300,000-600,000 units/mg protein
• Poids mol.: 11068 by amino acid sequence
• Technique(s): cell based assay: suitable
• Adéquation: suitable for separating native or denatured proteins, or nucleic acids
• Application(s): cell analysis
• Température de stockage: 2-8°C

Description

Application

Ribonuclease T1 (RNase T1) from Aspergillus oryzae is used to digest denatured RNA prior to sequencing and is used for protein folding studies .

Actions biochimiques/physiologiques

Ribonuclease T1 (RNase T1) from Aspergillus oryzae is an endoribonuclease that hydrolyzes after G residues. Cleavage occurs between the 3′-phosphate group of a guanidine ribonucleotide and 5′-hydroxyl of the adjacent nucleotide. The initial product is a 2′:3′ cyclic phosphate nucleoside that is hydrolyzed to the corresponding 3′-nucleoside phosphate. It differs from Pancreatic RNase in that it attacks the guanine sites specifically to yield 3′-GMP and oligonucleotides with a 3′-GMP terminal group.

Définition de l'unité

One unit will produce acid soluble oligonucleotides equivalent to a ΔA₂₆₀ of 1.0 in 15 min at pH 7.5 at 37°C, in a reaction volume of 1.0 mL. Substrate: Yeast RNA.

Forme physique

Suspension in 2.8 M (NH₄)₂SO₄ solution

Remarque sur l'analyse

Protein determined by E1%/280

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves