P8215
Protease Inhibitor Cocktail
DMSO solution, for the inhibition of serine, cysteine, aspartic and metalloproteases, for use with fungal and yeast extracts, DMSO solution
Synonyme(s) :
Protease Inhibitor Mix, Protease inhibitor
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About This Item
Produits recommandés
product name
Cocktail d'inhibiteurs de protéases, for use with fungal and yeast extracts, DMSO solution
Niveau de qualité
Forme
DMSO solution
Température de stockage
−20°C
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Description générale
The Protease Inhibitor Cocktail for fungal and yeast extracts is a mixture of protease inhibitors in DMSO solution. The cocktail has been optimized and tested for use on fungal and yeast samples, specifically on Saccharomyces cerevisiae cells. One mL of the cocktail is recommended for the inhibition of proteases extracted from 20 g of yeast.
Spécificité
Inhibits serine, cysteine, aspartic, and metalloproteases.
Application
Protease Inhibitor Cocktail has been used as a component in:
- FA buffer to wash spheroplasts to obtain an average DNA fragment size for chromatin immunoprecipitation (chip)
- potassium phosphate buffer (pbs) to digest chitin
- extraction buffer for grinding powdered mycelia for co-immunoprecipitation analysis
Caractéristiques et avantages
- Broad specificity: inhibits a wide range of proteases, providing comprehensive protection to fungal and yeast extracts.
- Tested on Saccharomyces cerevisiae cells: the cocktail has been optimized for use on this commonly studied yeast strain.
- Convenient packaging: available in a 1 or 5 mL glass bottle for easy handling and storage.
- Ready-to-use solution: the cocktail is supplied in DMSO solution for immediate use in protease inhibition assays.
- Effective inhibition: each component in the cocktail has been carefully selected for its specific inhibitory properties, ensuring reliable and consistent results.
Composants
AEBSF, 100 mM
E-64, 1.4 mM
Pepstatin A, 2.2 mM
1,10-Phenanthroline, 500 mM
E-64, 1.4 mM
Pepstatin A, 2.2 mM
1,10-Phenanthroline, 500 mM
Autres remarques
Mixture of protease inhibitors with broad specificity for the inhibition of serine, cysteine, aspartic and metallo-proteases. Contains 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF), pepstatin A, E-64, and 1,10-phenanthroline.
This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
Quantité
One mL is recommended for the inhibition of proteases extracted from 20 g of yeast.
Forme physique
Solution in DMSO
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
188.6 °F
Point d'éclair (°C)
87 °C
Certificats d'analyse (COA)
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