P6635

Phosphorylase b from rabbit muscle

lyophilized powder, ≥20 units/mg protein, 2× crystallization

• Synonyme(s) : α-Glucan Phosphorylase, 1,4-α-D-Glucan:orthophosphate α-D-glucosyltransferase, Glycogen Phosphorylase
• Numéro CAS: 9012-69-5
• Numéro de classification (Commission des enzymes): 2.4.1.1 (BRENDA, IUBMB)
• Numéro CE : 232-737-0
• Numéro MDL: MFCD00131905
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.77

Propriétés

• Source biologique: rabbit muscle
• Niveau de qualité: 300
• Forme: lyophilized powder
• Activité spécifique: ≥20 units/mg protein
• Poids mol.: 97,200 Da by calculation
• Produit purifié par: 2× crystallization
• Conditions de stockage: (Keep container tightly closed in a dry and well-ventilated place)
• Technique(s): mass spectrometry (MS): suitable
• Impuretés: ~0.01 μmol/mg protein 5′-AMP (This low level will not interfere with phosphorylase and phosphorylase kinase assays.)
• Numéro d'accès UniProt: A0A5F9CLZ9; A0A5F9CRR0; A0A5F9D1C4; A0A5F9DLL9
• Activité étrangère: phosphoglucomutase ≤1.0%; phosphorylase a ≤10%; phosphorylase kinase ≤0.5%; phosphorylase phosphatase, debrancher enzyme, AMPase and ATPase ≤0.1%
• Température de stockage: −20°C

Description

Description générale

Research area: Cell Signaling

Glycogen phosphorylase (PG), a specialized complex allosteric enzyme has an evolutionarily conserved gene sequence. GP contains a family of three isozymes such as muscle GP (mGP), liver GP (lGP), and brain GP (bGP) in humans.

Application

Phosphorylase b from rabbit muscle has been used:

• in the calibration of Sepharose C1-6B columns while studying the molecular weight of methylamine dehydrogenase subunits
• in ion mobility-mass spectrometry studies of phosphorylase B ions that have been generated with supercharging reagents, in the charge-reducing buffer
• for the preparation of p³² labeled phosphorylase A using phosphorylase kinase and [³²P]ATP
• in phosphorylase phosphatase assay
• in enzyme assay as a positive control to ensure the reaction system for the activity determination was adopted

Actions biochimiques/physiologiques

Phosphorylase b is a non-active form and is present in resting muscles. Phosphorylase b kinase activity increases significantly when the Mg²⁺:ATP ratio exceeds 1. The breakdown of ATP during muscle contraction is thought to trigger in vivo conversion of phosphorylase b into a. Phosphorylase b is activated by inosine monophosphate. Glycogen phosphorylase (PG) enzyme plays a vital role in the first step of glycogenolysis. In the initial stage of glycogenolysis, glycogen phosphorylase(GP) breaks α-1,4- -glycosidic bonds, releasing the glucose-1-phosphate (G1P)molecule. When incubated with the proper concentrations of glucose-1-phosphate without the addition of primer, rabbit muscle phosphorylase B was found to be capable of forming protein-bound alpha-1,4 glucosyl chains.

Conditionnement

Package size based on protein content.

Définition de l'unité

One unit will form 1.0 μmole of α-D-glucose 1-phosphate from glycogen and orthophosphate in the presence of 5′-AMP, per min at pH 6.8 at 30 °C measured in a system containing phosphoglucomutase, NADP, and glucose 6-phosphate dehydrogenase. (One μmolar unit is equivalent to approx. 45 Cori units.)

Forme physique

Lyophilized powder containing lactose, 5′-AMP, and Mg(OAc)₂ (10 μmole per 100 mg protein)

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)