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Principaux documents

P3430

Sigma-Aldrich

Monoclonal Anti-Phosphoserine antibody produced in mouse

clone PSR-45, ascites fluid

Synonyme(s) :

Monoclonal Anti-Phosphoserine, Phospho Ser, Phospho serine, Phospho−Ser, Phospho−serine

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.44

Source biologique

mouse

Niveau de qualité

Conjugué

unconjugated

Forme d'anticorps

ascites fluid

Type de produit anticorps

primary antibodies

Clone

PSR-45, monoclonal

Contient

15 mM sodium azide

Technique(s)

indirect ELISA: 1:4,000
western blot: 1:500-1:1,000

Isotype

IgG1

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

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Description générale

Monoclonal Anti-Phosphoserine (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Spécificité

By ELISA and dot blot, the antibody reacts specifically with phosphorylated serine, both as free amino acid or conjugated to carriers such as BSA or KLH. No cross-reactivity is observed with non-phosphorylated serine, phosphothreonine, phosphotyrosine, AMP or ATP. This antibody has been used in immunoblotting for the localization of some phosphoserine-containing proteins. Certain proteins known to contain phosphorylated serine may not be recognized by this antibody due to steric hindrance of the recognition site.

Immunogène

phosphoserine conjugated to Keyhole Limpet Hemocyanin (KLH).

Application

Anti-Phosphoserine antibody may be used for indirect ELISA at a working dilution of 1:4000. For immunoblotting using rat brain cortex extracts, a working dilution of 1:500-1:1100 may be used. The antibody was used for immunoblotting to detect proteins phosphorylated at serine in stem extracts of Arabidopsis thalliana at a working dilution of 1:250.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western blotting following immunoprecipitation (1 paper)
Monoclonal Anti-Phosphoserine antibody produced in mouse has been used in western blotting.

Actions biochimiques/physiologiques

Phosphoserine (PS) can be used as a template to detect cancer antigen 125 (CA 125).

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Sapan J Patel et al.
Journal of cellular and molecular medicine, 21(3), 456-466 (2016-09-30)
B-cell novel protein-1 (BCNP1) or Family member of 129C (FAM129C) was identified as a B-cell-specific plasma-membrane protein. Bioinformatics analysis predicted that BCNP1 might be heavily phosphorylated. The BCNP1 protein contains a pleckstrin homology (PH) domain, two proline-rich (PR) regions and
Identification of cellulose synthase AtCesA7 (IRX3) in vivo phosphorylation sites?a potential role in regulating protein degradation
Taylor NG
Plant Molecular Biology, 64(1-2), 161-171 (2007)
Vinculin but not alpha-actinin is a target of PKC phosphorylation during junctional assembly induced by calcium
Perez-MM, et al.
Journal of Cell Science, 111(23), 3563-3571 (1998)
Pierrick Dudognon et al.
FEBS letters, 561(1-3), 44-50 (2004-03-12)
Endoplasmic reticulum (ER)-to-Golgi transport is blocked in mammalian cells during mitosis; however, the mechanism underlying this blockade remains unknown. Since COPII proteins are involved in this transport pathway, we investigated at the biochemical level post-translational modifications of COPII components during
Neil G Taylor
Plant molecular biology, 64(1-2), 161-171 (2007-04-12)
Cellulose is central to plant development and is synthesised at the plasma membrane by an organised protein complex that contains three different cellulose synthase proteins. The ordered assembly of these three catalytic subunits is essential for normal cellulose synthesis. The

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