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N5759

Sigma-Aldrich

4-Nitrophenyl β-D-cellobioside

chromogenic, ≥98% (TLC), powder

Synonyme(s) :

p-Nitrophenyl β-D-cellobioside

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About This Item

Formule empirique (notation de Hill):
C18H25NO13
Numéro CAS:
3482-57-3
Poids moléculaire :
463.39
Numéro Beilstein :
100234
Numéro MDL:
MFCD00069845
Code UNSPSC :
12352204
ID de substance PubChem :
24897749
Nomenclature NACRES :
NA.32

product name

4-Nitrophenyl β-D-cellobioside, ≥98% (TLC)

Niveau de qualité

300

Pureté

≥98% (TLC)

Forme

powder

Solubilité

water: 49.00-51.00 mg/mL

Température de stockage

2-8°C

Chaîne SMILES 

OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)[C@@H](O[C@@H]2CO)Oc3ccc(cc3)[N+]([O-])=O)[C@H](O)[C@@H](O)[C@@H]1O

InChI

1S/C18H25NO13/c20-5-9-11(22)12(23)14(25)18(30-9)32-16-10(6-21)31-17(15(26)13(16)24)29-8-3-1-7(2-4-8)19(27)28/h1-4,9-18,20-26H,5-6H2/t9-,10-,11-,12+,13-,14-,15-,16-,17-,18+/m1/s1

Clé InChI

IAYJZWFYUSNIPN-KFRZSCGFSA-N

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Application

4-Nitrophenyl β-D-cellobioside has been used as a substrate to assay β-D-celluliosidase or cellobiohydrolase (exocellulase) activity in the microbial community. It has also been used as a substrate to study the endoglucanase J30 activity.

Actions biochimiques/physiologiques

4-Nitrophenyl β-D-cellobioside is a cellotriose analog, and a chromogenic substrate commonly used for the detection of cellulase activity. Exoglucanase, endoglucanase and β -glucosidase hydrolyze 4-nitrophenyl β-D-cellobioside to form p-nitrophenol (PNP).

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

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R M Gardner et al.
Journal of bacteriology, 169(10), 4581-4588 (1987-10-01)
An exo-beta-1,4-glucanase (Exo A) from Ruminococcus flavefaciens FD-1 was purified to homogeneity and characterized. Enzyme activity was monitored during purification by using the substrate p-nitrophenyl-beta-D-cellobioside (NPC). Over 85% of the NPC activity was found to be extracellular once the filter
S J Han et al.
The Journal of biological chemistry, 270(43), 26012-26019 (1995-10-27)
Bacillus sp. D04 secreted a bifunctional cellulase that had a molecular weight of 35,000. This cellulase degraded Cm-cellulose, cellotetraose, cellopentaose, p-nitrophenyl-beta-D-cellobioside, and avicel PH101. Based on the high performance liquid chromatography analysis of the degradation products, this cellulase randomly cleaved
M Fukumura et al.
Bioscience, biotechnology, and biochemistry, 59(1), 47-50 (1995-01-01)
The thermal denaturation process of Clostridium stercorarium F-9 xylanase (XynB) was studied by monitoring remaining activity and recovered activity of the enzyme. At pH 5.5, aggregation occurred rapidly after the thermal denaturation initiated. The aggregated protein could be dissolved in
Effects of microplastic fibers on soil aggregation and enzyme activities are organic matter dependent
Liang Y, et al.
Frontiers in environmental science., 97 null
Keum Shin et al.
Journal of microbiology and biotechnology, 20(12), 1681-1688 (2011-01-05)
A screening for cellobiohydrolase (CBH) activity was performed and Fomitopsis pinicola KMJ812 was selected for further characterization as it produced a high level of CBH activity. An extracellular CBH was purified to homogeneity by sequential chromatography of F. pinicola culture
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