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M7292

Sigma-Aldrich

M16 Medium

With sodium bicarbonate and lactic acid, without penicillin and streptomycin, liquid, sterile-filtered

Synonyme(s) :

Cell Culture Medium, Cell Culture Medium M16, M16 Medium Solution

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75

Niveau de qualité

Stérilité

sterile-filtered

Forme

liquid

Technique(s)

cell culture | embryo: suitable

Impuretés

endotoxin, tested

Composants

NaHCO3: 2.101 g/L
phenol red: 0.0106 g/L
sodium pyruvate: 0.0363 g/L
glucose: 1.0 g/L (Dextro)

Conditions d'expédition

ambient

Température de stockage

2-8°C

Application

M2 and M16 Medium are common media for in vitro culture of preimplantation stage embryos. It is a modified Krebs-Ringer bicarbonate solution, which is very similar to Whitten′s Medium. M16 contains pyruvate and lactate as energy sources since preimplantation embryos do not utilize glucose efficiently.

Qualité

Presence of particulates in solution will not affect product′s performance.

Reconstitution

Supplement with 0.06 g/L potassium penicillin-G and 0.05 g/L streptomycin sulfate.

Remarque sur l'analyse

This product is tested for its ability to support the development of one-cell mouse embryos to expanded blastocysts. Minimum requirement is 80% development to blastocyst.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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