M5524
Murashige and Skoog Basal Salt Mixture (MS)
powder, suitable for plant cell culture
Synonyme(s) :
MS Basal Salts, MS0 Basal Salts, MSO Basal Salts
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About This Item
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.72
Produits recommandés
Forme
powder
Niveau de qualité
Technique(s)
cell culture | plant: suitable
Application(s)
agriculture
Conditions d'expédition
ambient
Température de stockage
2-8°C
Description générale
Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and Kinetin (cytokinin/cell division promoter).
Application
Murashige and Skoog Basal Salt Mixture (MS) has been used to support the growth and regeneration of transformed tobacco cells. It has also been used as a growth medium for germination of Arabidopsis thaliana (Col-0) seeds.
Variante de formule
Quantité
Formulated to contain 4.3 grams of powder per liter of medium.
Notes préparatoires
Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog Salt mixture (M5524) contains the macronutrients and micronutrients of the original classic formulation. It can be combined with M&S vitamins or Gamborg′s vitamins and supplemented with sucrose, agar, auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.
Autres remarques
Storage
Powdered media are extremely hygroscopic and must be protected from atmospheric moisture. If possible the entire contents of each package should be used immediately after opening.
Store dry medium in a desiccator at 0-5 °C.
Deterioration of powdered medium may be recognized by: 1) color change; 2) granulation, clumping, or particulate matter throughout the powder; 3) insolubility; 4) pH change; or 5) inability to promote growth when properly used.
Media Preparation
Powdered media are extremely hygroscopic and must be protected from atmospheric moisture. If possible the entire contents of each package should be used immediately after opening.
Store dry medium in a desiccator at 0-5 °C.
Deterioration of powdered medium may be recognized by: 1) color change; 2) granulation, clumping, or particulate matter throughout the powder; 3) insolubility; 4) pH change; or 5) inability to promote growth when properly used.
Media Preparation
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Ox. Sol. 3
Code de la classe de stockage
5.1B - Oxidizing hazardous materials
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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