L7018
Lipopolysaccharides from Pseudomonas aeruginosa 10
purified by trichloroacetic acid extraction
Synonyme(s) :
LPS
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About This Item
Produits recommandés
Source biologique
Pseudomonas aeruginosa (10)
Niveau de qualité
Forme
lyophilized powder
Produit purifié par
trichloroacetic acid extraction
Impuretés
1-10% Protein (Lowry)
Couleur
white to yellow
Solubilité
water: 4.90-5.10 mg/mL, hazy to turbid, colorless to light yellow
Conditions d'expédition
ambient
Température de stockage
2-8°C
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Description générale
This product is TCA extracted from Pseudomonas aeruginosa serotype 10. The source strain is ATCC 27316.
Application
Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).
Actions biochimiques/physiologiques
Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.
Notes préparatoires
The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.
Autres remarques
To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
Certificats d'analyse (COA)
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