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Key Documents

HT10132

Sigma-Aldrich

Bouin′s solution

histological fixative

Synonyme(s) :

Bouin’s fluid

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About This Item

Numéro MDL:
MFCD00146169
Code UNSPSC :
12171500
Nomenclature NACRES :
NA.47

Niveau de qualité

200

Durée de conservation

Expiry date on the label

Composition

acetic acid, 5%
formaldehyde, 9%
picric acid, 0.9%

Couleur

yellow

Application(s)

hematology
histology

Température de stockage

room temp

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Description générale

Bouin′s solution is a noncoagulant yellow stain fixative that consists of saturated picric acid, formaldehyde, and acetic acid.

Application

Bouin′s solution is an excellent fixative for preserving soft and delicate structures. It is used as a mordant in various trichrome procedures.
Additionally, it has been used for the following purposes-
  • staining of human liver biopsy tissue samples for two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) imaging
  • fixation of rat ovaries for the observing follicle cells using microscopy
  • fixation of gonads of crab for morphological and histological analysis
Prepared with saturated picric acid, formaldehyde and acetic acid. Excellent fixative for preserving soft and delicate structures. Used as a mordant in various trichrome procedures.

Actions biochimiques/physiologiques

Bouin′s solution causes shrinkage of tissues when used as a preservative due to its picric acid component. It preserves morphological and nuclear information in tissues upon fixation.

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

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Cengiz Yildiz et al.
Cryobiology, 84, 4-9 (2018-09-10)
Grafting of cryopreserved testicular tissue is a promising tool for fertility and testicular function preservation in endangered species, mutant animals, or cancer patients for future use. In this study, we aimed to improve the whole neonatal mouse testicular tissue cryopreservation
Won-Young Lee et al.
Animal reproduction science, 182, 1-8 (2017-05-04)
Spermatogenesis begins at puberty and continues throughout a male's life. This process is initiated and maintained by spermatogonial stem cells in the seminiferous tubules, and these cells produce haploid spermatozoa. Markers of male germ cells have been fully identified in
Suzana Ulian-Benitez et al.
PLoS genetics, 13(8), e1006968-e1006968 (2017-08-29)
Neurotrophism, structural plasticity, learning and long-term memory in mammals critically depend on neurotrophins binding Trk receptors to activate tyrosine kinase (TyrK) signaling, but Drosophila lacks full-length Trks, raising the question of how these processes occur in the fly. Paradoxically, truncated
Roman Leontovyč et al.
Scientific reports, 9(1), 1347-1347 (2019-02-06)
Trichobilharzia species are parasitic flatworms (called schistosomes or flukes) that cause important diseases in birds and humans, but very little is known about their molecular biology. Here, using a transcriptomics-bioinformatics-based approach, we explored molecular aspects pertaining to the nutritional requirements
Hua Guo et al.
Developmental biology, 430(1), 214-223 (2017-08-16)
GW182 (also known asTNRC6) family members are critically involved in the final effector phase of miRNA-mediated mRNA repression. The three mammalian paralogs, TNRC6a, b and c, are thought to be redundant based on Argonaute (Ago) binding, tethering assays, and RNAi
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