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Key Documents

GE28-9782-43

HiScreen™ Blue Fast Flow

Cytiva 28-9782-43

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About This Item

Code UNSPSC :
13111000
Nomenclature NACRES :
NA.56

ligand

Cibacron Blue

Conditionnement

pkg of 1 ea

Fabricant/nom de marque

Cytiva 28-9782-43

Conditions de stockage

(20% Ethanol, 0.1 M potassium phosphate)
 (20% Ethanol, 0.9% NaCl, 1 mM Ca2₊ and 1 mM Mn2₊)

Paramètres

1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi

Taille du lit

7.7 mm × 100 mm

Volume du lit

4.7 mL

D.I. de la colonne

7.7 mm

Matrice

6% cross-linked agarose

Taille des particules

45-165 μm

Diamètre moyen

90 μm

cleaning

3-13

working range

4-12

Adéquation

suitable for bioprocess medium

Température de stockage

2-8°C

Description générale

HiScreen™ columns prepacked with Blue Sepharose™ Fast FLow are an excellent choice for method optimization and parameter screening for purification of albumin, enzymes (including NAD+ and NADP+), coagulation factors, interferons, and related proteins.

Application

HiScreen™ columns are part of the process development platform available from Cytiva. The columns are prepacked with a range of BioProcess chromatography media (resin) and designed for method optimization and parameter screening. HiScreen™ columns have small bed volumes (4.7 ml), reducing the cost of sample and buffer consumption. The media used in HiScreen™ columns are also available in other prepacked formats and as bulk packs, for all scales of work from development and pilot studies to routine production.

HiScreen™ Blue FF is prepacked with the BioProcess chromatography medium Blue Sepharose™ 6 Fast Flow. This medium has Cibacron Blue 3G covalently attached to the Sepharose™ 6 Fast FLow matrix by the triazine coupling method. The Blue dye binds many Proteins, such as albumin, interferon, lipoProteins, and blood coagulation factors. It also binds several enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors (e.g., NAD).

Caractéristiques et avantages

  • Can be used for depletion of albumin.
  • Prepacked, ready-to-use columns for convenient process development.
  • Excellent choice for method optimization and parameter screening due to the 10 cm bed height.
  • Easy connection in series to achieve 20 cm bed height.
  • Small bed volume gives fast results and minimal sample/buffer consumption
  • Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

HiScreen is a trademark of Cytiva
Sepharose is a trademark of Cytiva

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

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