GE28-9356-04

HiPrep^™ 16/60 Sephacryl^® S-400 HR

Cytiva 28-9356-04, column L × I.D. 60 cm × 16 mm, 50 μm avg. part. size

• Synonyme(s) : HiPrep Chromatography Column, Sephacryl S-400 HR
• Code UNSPSC : 41115700
• Nomenclature NACRES : SB.52

Propriétés

• Matériaux: polypropylene (PP)
• Poids mol.: 20000-8000000 Da (globular proteins)
• Conditionnement: pkg of 1 ea
• Fabricant/nom de marque: Cytiva 28-9356-04
• Paramètres: <0.5 mL/min flow rate; <5 mL sample volume; 0.15 Mpa (22 psi)
• Volume du lit: 120 mL
• Colonne, L × D.I.: 60 cm × 16 mm
• Matrice: dextran-acrylamide particle platform; allyl dextran and N, N’-methylenebisacrylamide; polymeric
• Groupe de la matrice active: dextran-acrylamide phase
• Taille moy. des particules: 50 μm
• cleaning: 2-13
• working range: 3-11
• Technique de séparation: size exclusion (SEC)

Description

Description générale

HiPrep^™ 16/60 Sephacryl^® S-400 HR is a prepacked gel filtration column for preparative separation of biomolecules in the molecular weight range of 20 000 to 8 000 000 with sample volumes up to 5 mL.

Application

Sephacryl^® S-400 is optimized for separating polysaccarides and other macromolecules with extended structures e.g. proteoglycans and liposomes.

Caractéristiques et avantages

• Gel filtration columns for low to medium pressure AKTA systems.
• The HiPrep^™ Sephacryl^® series offer reproducible separations over a wide molecular weight range.
• Convenient, prepacked HiPrep^™ Sephacryl^® columns speed up protein purification.
• Maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability resulting from the hydrophilic, rigid allyl dextran/ bisacrylamide matrix.

Adéquation

All commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile (tested at 40oC for 1 week), 0.5 M NaOH (only for cleaning-in-place)

gel filtration columns for low to medium pressure ÄKTA systems+AE14

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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Informations légales

HiPrep is a trademark of Cytiva

Sephacryl is a registered trademark of Cytiva

Informations sur la sécurité

• Pictogrammes: GHS02
• Mention d'avertissement: Warning
• Mentions de danger: H226
• Conseils de prudence: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Code de la classe de stockage: 3 - Flammable liquids