G4134
Glucose-6-phosphate déshydrogénase from baker′s yeast (S. cerevisiae)
Type IX, lyophilized powder, 200-400 units/mg protein (modified Warburg-Christian)
Synonyme(s) :
G-6-P-DH, Zwischenferment
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Produits recommandés
Type
Type IX
Niveau de qualité
Forme
lyophilized powder
Activité spécifique
200-400 units/mg protein (modified Warburg-Christian)
Poids mol.
128 kDa
Teneur en β-NADP et β-NADPH
≤10 mmol/mol
Application(s)
agriculture
Conditions d'expédition
dry ice
Température de stockage
−20°C
Vous recherchez des produits similaires ? Visite Guide de comparaison des produits
Catégories apparentées
Description générale
Research area: Cell Signaling
Glucose-6-phosphate dehydrogenase (G6PD) is a key metabolic enzyme of the pentose phosphate pathway. In S. cerevisiae, it is encoded by the ZWF1 gene. G6PD exists as a tetramer in its active form.
Glucose-6-phosphate dehydrogenase (G6PD) is a key metabolic enzyme of the pentose phosphate pathway. In S. cerevisiae, it is encoded by the ZWF1 gene. G6PD exists as a tetramer in its active form.
Application
Glucose-6-phosphate dehydrogenase is used:
- To test ketose reductase activity in developing maize endosperm.
- For recycling microassay of β-NADP and β-NADPH.
- To measure the intracellular levels of NADPH and total NADP.
- To measure the nicotinamide adenine dinucleotide (NAD) kinase kinetic assay activity.
Actions biochimiques/physiologiques
La glucose-6-phosphate déshydrogénase catalyse la transformation du glucose-6-phosphate en 6-phosphogluconolacétone, ce qui constitue la première étape de la voie des pentoses phosphates.
Glucose-6-phosphate dehydrogenase catalyzes the rate-limiting step in the pentose phosphate pathway. Its function involves the conversion of glucose-6-phosphate to 6-phosphogluconolacetone while generating NADPH, which is essential for the regeneration of glutathione The glutathione system utilizes nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) to effectively eliminate excess hydrogen peroxide. Glucose-6-phosphate dehydrogenase (G6PD) plays an important role in regulating cell growth and survival. Their levels are higher in cells undergoing normal and neoplastic growth. Increased glucose-6-phosphate dehydrogenase activity plays a pivotal role in preventing reactive oxygen species mediated cell death. Glucose-6-phosphate dehydrogenase is over expressed in several cancers whereas its activity is reduced in hyperglycemia. A deficiency in glucose-6-phosphate dehydrogenase causes hemolysis.
Définition de l'unité
One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NADP at pH 7.4 at 25 °C.
Forme physique
Lyophilized powder essentially sulfate-free, containing approx. 20% sodium citrate
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Resp. Sens. 1
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Les clients ont également consulté
The global prevalence of glucose-6-phosphate dehydrogenase deficiency: A systematic review and meta-analysis
Nkhoma ET and Poole C
Blood Cells, Molecules and Diseases, 42, 267?278-267?278 (2009)
Impact of glucose-6-phosphate dehydrogenase deficiency on the pathophysiology of cardiovascular disease
Hecker PA, et al.
American Journal of Physiology. Heart and Circulatory Physiology, 304(4), H491-H500 (2013)
Glucose-6-phosphate dehydrogenase, NADPH, and cell survival
Stanton R C
IUBMB Life, 64(5), 362-369 (2012)
The Pentose Phosphate Pathway in Yeasts-More Than a Poor Cousin of Glycolysis
Bertels LK, et al.
Biomolecules, 11(5), 725-725 (2021)
D Oh et al.
Molecular and cellular biology, 10(4), 1415-1422 (1990-04-01)
The Saccharomyces cerevisiae GAL5 (PGM2) gene was isolated and shown to encode the major isozyme of phosphoglucomutase. Northern (RNA) blot hybridization revealed that the GAL5 transcript level increased three- to fourfold in response to galactose and was severely repressed in
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique