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F4143

Sigma-Aldrich

Anti-Mouse IgG (Fc specific)–FITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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About This Item

Numéro MDL:
MFCD00162628
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

goat

Niveau de qualité

200

Conjugué

FITC conjugate

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Conditions de stockage

protect from light

Technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
indirect immunofluorescence: 1:128

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

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Catégories apparentées

Description générale

IgG antibody is secreted by B cells and is found in blood and extracellular fluids and
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system.

Spécificité

Binds mouse IgG; does not bind other mouse Igs.

Immunogène

purified mouse IgG, Fc fragment

Application

Anti-Mouse IgG (Fc specific)-FITC antibody has been used in
  • immunohistochemistry
  • immunofluorescence
  • flow cytometry
  • immunocytochemistry
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Actions biochimiques/physiologiques

IgG provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections.

Autres remarques

Antibody adsorbed with bovine, equine and human serum proteins.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Notes préparatoires

Adsorbed to reduce background staining with bovine, horse or human samples.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Jiří Zahradník et al.
ACS synthetic biology, 10(12), 3445-3460 (2021-11-24)
Here, we enhanced the popular yeast display method by multiple rounds of DNA and protein engineering. We introduced surface exposure-tailored reporters, eUnaG2 and DnbALFA, creating a new platform of C and N terminal fusion vectors. The optimization of eUnaG2 resulted
Qiong Wu et al.
Frontiers in microbiology, 9, 1661-1661 (2018-08-09)
Escherichia coli is a common cause of mastitis in dairy cows. The adaptor protein apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) synergizes with caspase-1 to regulate inflammasome activation during pathogen infection. Here, the ASC gene was knocked out
P Xu et al.
Iranian journal of veterinary research, 22(1), 65-71 (2021-06-22)
Mammary epithelial cells (MECs) have been widely-used over the years as models to understand the physiological function of mammary disease. This study aimed to establish a culture system and elucidate the unique characteristics of bovine mammary epithelial cells (BMECs) from
Roberto Gozalbo-Rovira et al.
PLoS pathogens, 15(6), e1007865-e1007865 (2019-06-22)
Rotavirus is the leading agent causing acute gastroenteritis in young children, with the P[8] genotype accounting for more than 80% of infections in humans. The molecular bases for binding of the VP8* domain from P[8] VP4 spike protein to its
Patrick L Sinn et al.
Journal of virology, 77(10), 5902-5910 (2003-04-30)
The practical application of gene therapy as a treatment for cystic fibrosis is limited by poor gene transfer efficiency with vectors applied to the apical surface of airway epithelia. Recently, folate receptor alpha (FR alpha), a glycosylphosphatidylinositol-linked surface protein, was
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