E5529
EZview™ Red Streptavidin Affinity Gel
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About This Item
Produits recommandés
Forme
suspension
Niveau de qualité
Durée de conservation
1 yr
Technique(s)
western blot: suitable
Matrice
4% agarose
pH
7.2
Capacité
~10 μg(biotin per ml of packed gel)
Conditions d'expédition
wet ice
Température de stockage
2-8°C
Description générale
The EZ View Red Streptavidin Affinity gel is composed of streptavidin, covalently attached to
cyanogen bromide-activated 4% agarose beads. It is designed to capture (pull-down) the biotinylated target molecules, such as proteins, peptides, antibodies, nucleic acids, lectins, receptors and ligands.
cyanogen bromide-activated 4% agarose beads. It is designed to capture (pull-down) the biotinylated target molecules, such as proteins, peptides, antibodies, nucleic acids, lectins, receptors and ligands.
Application
Sutitable for use with immunoprecipitation, western blotting,and enzyme assays.
When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduce the risk of pellet loss. EZview™ resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.
Caractéristiques et avantages
- Increased visibility - Red color reduces risk of incidental aspiration
- Improved recovery of target protein by reduced accidental loss
- Higher reproducibility - More consistent yields
Forme physique
1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon
Informations légales
EZview is a trademark of Sigma-Aldrich Co. LLC
Code de la classe de stockage
10 - Combustible liquids
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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