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Key Documents

E4142

Sigma-Aldrich

ExtrAvidin®−Cy3

buffered aqueous solution

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About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Conjugué

CY3 conjugate

Niveau de qualité

Forme

buffered aqueous solution

Ampleur du marquage

3-9 mol Cy3 per mol protein

Technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Application

ExtrAvidin®-Cy3 is suitable for the following applications:
  • Immunohistochemistry (formalin-fixed, paraffin-embedded sections) at a dilution of 1:100
  • Immunocytochemistry.
  • Used for labeling.

Actions biochimiques/physiologiques

Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. It consists of four high affinity binding sites for biotin. ExtrAvidin® is prepared from egg white avidin. It is a modified form of affinity purified avidin and binds biotin with high specific activity of avidin with the low background staining of streptavidin, a biotin binding protein produced by the bacteria Streptomyces avidinii. ExtrAvidin® is covalently conjugated to Cy3 dye and is used for various techniques.

Forme physique

Solution in 0.01 M phosphate buffered saline, containing 1% bovine serum albumin and 15 mM sodium azide

Notes préparatoires

Affinity purified protein

Informations légales

Cy3 is a trademark of Cytiva
ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Andrea-Romana Prusa et al.
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