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Merck
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Principaux documents

E3024

Sigma-Aldrich

Earle′s Balanced Salts

With sodium bicarbonate, without phenol red, liquid, sterile-filtered, suitable for cell culture

Synonyme(s) :

EBSS

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About This Item

Numéro MDL:
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75

Niveau de qualité

Stérilité

sterile-filtered

Forme

liquid

Technique(s)

cell culture | mammalian: suitable

Impuretés

endotoxin, tested

Composants

phenol red: no
NaHCO3: 2.2 g/L
glucose: 1.0 g/L (Dextro)

Conditions d'expédition

ambient

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Description générale

Earle′s balanced salts solution (EBSS) is a saline solution with physiological pH that stimulates autophagy. It augments Bacillus Calmette-Guérin (BCG)-induced dendritic cells (DCs) by upregulating cluster of differentiation (CD86), human leukocyte antigen-DR isotype (HLA-DR), and interleukin (IL-6).

Application

Earle′s balanced salts has been used to incubate filter-grown monolayers of Caco-2 cells to induce starvation, along with HEPES buffer to detect NADPH oxidase activity in trypsinized cells. It has also been used to treat CEM RES cells and perform amino acid depletion experiment.

Autres remarques

Phenol red has been shown to interfere with the growth of some cells at cloning densities. Use this medium when working with stem cells or when growing cells at low densities.

Supplément

Réf. du produit
Description
Tarif

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Consulter la Bibliothèque de documents

Morgan Wong et al.
American journal of physiology. Cell physiology, 316(5), C753-C765 (2019-03-21)
A defective tight junction (TJ) barrier is a key pathogenic factor for inflammatory bowel disease. Previously, we have shown that autophagy, a cell survival mechanism, enhances intestinal epithelial TJ barrier function. Autophagy-related protein-6 (ATG6/beclin 1), a key protein in the
Natthakan Thongon et al.
Cancer & metabolism, 6, 1-1 (2018-03-16)
Inhibitors of nicotinamide phosphoribosyltransferase (NAMPT), the rate-limiting enzyme in NAD+ biosynthesis from nicotinamide, exhibit anticancer effects in preclinical models. However, continuous exposure to NAMPT inhibitors, such as FK866, can induce acquired resistance. We developed FK866-resistant CCRF-CEM (T cell acute lymphoblastic
Léon-Charles Tranchevent et al.
Genome research, 27(6), 1087-1097 (2017-04-20)
Transcriptomic genome-wide analyses demonstrate massive variation of alternative splicing in many physiological and pathological situations. One major challenge is now to establish the biological contribution of alternative splicing variation in physiological- or pathological-associated cellular phenotypes. Toward this end, we developed
Jagadeesan Arunkumar et al.
Microbial pathogenesis, 118, 301-309 (2018-04-01)
Herpes simplex virus - 2 (HSV-2) causes lifelong persisting infection in the immunocompromised host and intermittent in healthy individuals with high morbidity in neonatals and also increase the transmission of HIV. Acyclovir is widely used drug to treat HSV-2 infection
Joon Hee Kang et al.
Biomolecules & therapeutics, 27(1), 34-40 (2018-09-21)
Transglutaminase 2 (TGase 2) plays a key role in p53 regulation, depleting p53 tumor suppressor through autophagy in renal cell carcinoma. We found that microtubule-associated protein 1A/1B-light chain 3 (LC3), a hallmark of autophagy, were tightly associated with the level

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