E2636
ExtrAvidin® –Alkaline Phosphatase
buffered aqueous solution
Synonyme(s) :
Alkaline Phosphatase Reagent, ExtrAvidin Alkaline Phosphatase
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About This Item
Produits recommandés
Conjugué
alkaline phosphatase conjugate
Niveau de qualité
Forme
buffered aqueous solution
Technique(s)
dot blot: 1:300,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tissues sections
indirect ELISA: 1:60,000
Conditions d'expédition
ambient
Température de stockage
2-8°C
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Description générale
Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. It consists of four high affinity binding sites for biotin. ExtrAvidin® is prepared from egg white avidin. It is a modified form of affinity purified avidin with high specific activity of avidin and low background staining of streptavidin. It is a biotin binding protein produced by the bacteria Streptomyces avidinii. ExtrAvidin has been conjugated to Alkaline Phosphatase and is used for various techniques.
Application
ExtrAvidin™ -Alkaline Phosphatase has been used in:
- Dot blot
- Immunohistochemistry (formalin-fixed, paraffin-embedded sections)
- Indirect ELISA
- Enzyme linked immunosorbent assay (ELISA)
- Enzyme-linked immunosorbent spot (ELISPOT) analysis
- Competitive ELISA
Actions biochimiques/physiologiques
Alkaline Phosphatase catalyzes the hydrolysis of esters to yield phosphoric acid. It is also facilitate the transport of substances across membranes.
Forme physique
Solution in 0.05 M Tris-HCl buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin and 15 mM sodium azide
Notes préparatoires
Affinity purified protein
Informations légales
ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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