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DUO96010

Sigma-Aldrich

Duolink® PLA Multicolor Probemaker Kit - Red

Proximity Ligation Assay Multiplexing: protein-protein interaction, post-translational modification and low abundant protein events

Synonyme(s) :

in situ Proximity Ligation Assay Kit, Protein Protein Interaction Kit

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About This Item

Code UNSPSC :
41105331
Nomenclature NACRES :
NA.32

Gamme de produits

Duolink®

Technique(s)

immunofluorescence: suitable
multiplexing: suitable
proximity ligation assay: suitable

Fluorescence

λex 594 nm; λem 624 nm (Texas Red®)
λex 594 nm; λem 624 nm (red)

Adéquation

suitable for fluorescence

Conditions d'expédition

dry ice

Température de stockage

−20°C

Application

Specificity
Red fluorescence detection reagents are often used with Texas Red® filter.

Application Note
Primary antibodies are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC), or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells are required.

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Caractéristiques et avantages

Multicolor Duolink® proximity ligation assay (PLA®) is an application of the Duolink® technology that allows for endogenous, simultaneous detection of up to 4 protein interactions, post-translational modifications, or protein expression levels (including low abundant protein) in fixed cells and tissue samples. Follow the Duolink® PLA Probemaker Guide for Multicolor Detection and the Duolink® PLA Multicolor Detection protocol to use this product.

  • Visualize and detect multiple proteins events in one experiment.
  • No overexpression or genetic manipulation required
  • High specificity (fewer false positives)
  • Detection of low abundant proteins due to rolling circle amplification
  • Relative quantification possible
  • No special equipment needed
  • Faster and more simple than FRET
  • Increased accuracy compared to co-IP
  • Publication-ready results

Components
Duolink® PLA Multicolor Probemaker Kit - Red contains all the necessary reagents to perform the conjugations of Red Oligo A and Red Oligo B directly to primary antibodies. The resulting PLA probes (Red A and Red B) must be used together and cannot be paired with any other Duolink

This product is comprised of the following:
  • Red Oligo A DUO86010A
  • Red Oligo B DUO86010B
  • Conjugation Buffer DUO82033
  • Stop Reagent DUO82034
  • Storage Solution DUO82035

See datasheet for more information.

To perform a Multicolor Duolink® PLA experiment primary antibodies (PLA, IHC, ICC or IF validated) that recognize protein targets of interest are required. Duolink® PLA Multicolor Probemaker-generated probes (Red A/Red B, Green C/Green D, Orange F/Orange G, and/or FarRed H/FarRed I) are used in combination to perform the Multiplex experiment.The reagents in the Duolink® PLA Multicolor Reagent Pack are required to perform the amplification and detection of the resulting PLA probes. Required additional reagents include Duolink® Wash Buffers and Mounting Medium.

Informations légales

Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany
PLA is a registered trademark of Merck KGaA, Darmstadt, Germany
Texas Red is a registered trademark of Life Technologies

Pictogrammes

Exclamation markEnvironment
GHS07,GHS09

Mention d'avertissement

Warning

Mentions de danger

H317,H411

Classification des risques

Aquatic Chronic 2 - Skin Sens. 1

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Chatchai Phoomak et al.
Science advances, 7(3) (2021-02-02)
Asparagine (N)-linked glycosylation is required for endoplasmic reticulum (ER) homeostasis, but how this co- and posttranslational modification is maintained during ER stress is unknown. Here, we introduce a fluorescence-based strategy to detect aberrant N-glycosylation in individual cells and identify a

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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