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CS0390

Sigma-Aldrich

Mitochondria Staining Kit

1 kit sufficient for 40 tests (of 5 mL cell suspensions), 1 kit sufficient for 200 tests (of 1 mL cell suspensions)

Synonyme(s) :

JC-1 dye

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.32

Niveau de qualité

200

Utilisation

 kit sufficient for 200 tests (of 1 mL cell suspensions)
 kit sufficient for 40 tests (of 5 mL cell suspensions)

Conditionnement

pkg of 1 kit

Conditions de stockage

dry at room temperature

Technique(s)

flow cytometry: suitable
protein staining: suitable

Fluorescence

λex 490 nm; λem 530 nm (green) (JC-1 monomers)
λex 525 nm; λem 590 nm (red) (JC-1 aggregates)

Application(s)

cell analysis
detection

Méthode de détection

fluorometric

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Description générale

In normal cells, the JC-1 dye concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. Any event that dissipates the mitochondrial membrane potential (e.g. apoptosis) prevents the accumulation of the JC-1 dye in the mitochondria and thus, the dye is dispersed throughout the entire cell leading to a shift from red (JC-1-aggregates) to green fluorescence (JC-1 monomers). The fluorescence of the cells stained with this kit may be observed by fluorescence microscopy or measured by fluorimetric and flow cytometry analysis.

Application

The dissipation of the mitochondrial electrochemical potential gradient (Δψ) is known as an early event in apoptosis. This kit offers a fast and convenient method for the detection of changes in mitochondrial inner-membrane electrochemical potential in living cells using the cationic, lipophilic dye, JC-1.

Caractéristiques et avantages

Kit offers:
  • A fast, simple, and convenient method for staining and assaying mitochondria intactness
  • Contains all the reagents required for the detection of changes mitochondrial inner-membrane electrochemical potential
  • Contains the antibiotic valinomycin, which can be used as a control agent that prevents JC-1 aggregation
  • The shift in fluorescence is clearly detectable in both green and red channels
  • Suitable for adherent cells as well as cells in suspension
  • Has been tested with Jurkat, U-937, HeLa, NIH3T3 cells
  • The fluorescence signal can be observed by fluorescence microscopy or measured by flow cytometry

Composants de kit seuls

Réf. du produit
Description
  • DMSO 1 mL
  • JC Staining Buffer 5X 120 mL
  • JC-1 1 mg
  • Valinomycin Ready Made .1 mL

Code de la classe de stockage

10 - Combustible liquids

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Robert L Elliott et al.
PloS one, 14(4), e0214586-e0214586 (2019-04-02)
Many cells are cultured in media that contains an antibiotic to prevent bacterial contamination. Mycoplasma and other bacterial contamination is a serious problem for those involved in cell culture. Antibiotics in the media helps prevent this contamination and make life
Small Molecule Mediated Restoration of Mitochondrial Function Augments Anti-Mycobacterial Activity of Human Macrophages Subjected to Cholesterol Induced Asymptomatic Dyslipidemia.
Suman Asalla et al.
Frontiers in cellular and infection microbiology, 7, 439-439 (2017-10-27)
Whasun Lim et al.
Biology of reproduction, 95(4), 82-82 (2016-09-02)
Luteolin is a natural compound known for its anticancer effects on various human cancers by regulating signal transduction cascades. However, the effects of luteolin on human placental choriocarcinoma are not known. Results of the present study revealed that luteolin decreased
A Cossarizza et al.
Biochemical and biophysical research communications, 197(1), 40-45 (1993-11-30)
A new method for the cytofluorimetric analysis of mitochondrial membrane potential in intact cells has been developed by using the lipophilic cationic probe 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolcarbocyanine iodide (JC-1), whose monomer emits at 527 nm after excitation at 490 nm. Depending on the
Maria C Zanellati et al.
Frontiers in genetics, 6, 78-78 (2015-03-31)
Mutations in PARK2, encoding Parkin, cause an autosomal recessive form of juvenile Parkinson Disease (JPD). The aim of the present study was to investigate the impact of PARK2 mutations on mitochondrial function and morphology in human skin fibroblasts. We analyzed
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