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Key Documents

CLS3464

Corning® Costar® 24 well plates

8.0 μm pore size, Tissue Culture (TC)-treated surface flat bottom clear polystyrene wells, round wells, clear plate, PET membrane, sterile, case of 48 ea, pack of 12 ea

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About This Item

Code UNSPSC :
41122107
Nomenclature NACRES :
NB.13

product name

Corning® Costar® Transwell® cell culture inserts, TC treated, PET membrane, diam. 6.5 mm, pore size 8.0 μm, sterile

Matériaux

PET membrane
clear plate
flat bottom clear polystyrene wells
round wells

Stérilité

sterile

Caractéristiques

lid
skirt

Conditionnement

pack of 12 ea
case of 48 ea

Fabricant/nom de marque

Corning 3464

Diamètre

6.5 mm

Membrane, épaisseur × diam.

10 μm × 6.5 mm

Taille

24 wells

Volume de travail par puits

0.6 mL

Couleur

clear

Dimension de pores

8 μm
8.0 μm

Type de liaison

Tissue Culture (TC)-treated surface

Description générale

The Transwell® cell culture inserts feature a distinctive self-centered and hanging design that effectively prevents the unwanted wicking of medium between the insert and outer well. This in turn ensures the uninterrupted and intact co-culturing of cells in the lower compartment, allowing for undisturbed cell growth and maintenance.

Application

The Corning® Costar® Transwell® cell culture inserts are well-suited for cell staining applications and various chemotaxis assays, including fibroblast migration studies, tumor invasion and metastasis, trans-migrational studies, and invasion inhibition research. The inserts have been used in:
  • Transwell assay to study the role of TGF-β-MTA1-SMAD7-SMAD3-SOX4-EZH2 signaling axis in hepatocellular carcinoma cells
  • MTT Assay to study GAS5-mediated signaling in osteosarcoma cells
  • tri-cultured neuroinflammation model

Caractéristiques et avantages

  • 10 μm thick transparent polyester membrane
  • Treated for optimal cell attachment
  • Sterilized by gamma radiation Lids included TC-treated surface 0.33 cm2 cell growth area
  • Can be coated with a diverse range of extracellular matrices (ECM) and basement membrane extracts (BME)
  • Optical clarity enables clear visualization of cell outlines when observed under phase contrast microscopy
  • Offers compatibility with histochemical stains and fluorochromes for various cell staining applications

Informations légales

Corning is a registered trademark of Corning, Inc.
Costar is a registered trademark of Corning, Inc.
Transwell is a registered trademark of Corning, Inc.

Certificats d'analyse (COA)

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The CtBP1-HDAC1/2-IRF1 transcriptional complex represses the expression of the long noncoding RNA GAS5 in human osteosarcoma cells
Zhang X, et al.
International journal of biological and chemical sciences (2019)
Yan-Fang Zheng et al.
Journal of neurochemistry, 156(2), 249-261 (2020-09-06)
Neuroinflammation is believed to play a primary role in the pathogenesis of most neurodegenerative diseases including Alzheimer's disease, Parkinson's disease and schizophrenia. Currently, suitable in vitro neuroinflammation models for studying cellular interactions and inflammatory mechanisms at the neurovascular unit are
Aritro Nath et al.
Molecular cancer research : MCR, 19(2), 240-248 (2020-10-28)
Elevated uptake of saturated fatty acid palmitate is associated with metastatic progression of cancer cells; however, the precise signaling mechanism behind the phenomenon is unclear. The loss of cell adhesion proteins, such as desmoplakin (DSP), is a key driving event
Kangjun Zhang et al.
Cancer management and research, 13, 7087-7099 (2021-09-18)
Enhancer of zeste homolog 2 (EZH2) is implicated in hepatocellular carcinoma (HCC), but whether transforming growth factor-β (TGF-β)-metastasis associated 1 (MTA1)-SMAD7-SMAD3-SRY-Box Transcription Factor 4 (SOX4)-EZH2 signaling axis, in which EZH2 participates, is also involved in HCC remained unknown. Data on
Rodrigo Berzaghi et al.
Cancers, 11(5) (2019-05-22)
The abilities of cancer-associated fibroblasts (CAFs) to regulate immune responses in the context of radiotherapy remain largely unknown. This study was undertaken to determine whether ionizing radiation alters the CAF-mediated immunoregulatory effects on macrophages. CAFs were isolated from freshly-resected non-small
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