CGP1
Glutathione Peroxidase Cellular Activity Assay Kit
Sufficient for 100 colorimetric tests
Synonyme(s) :
Cellular glutathione peroxidase, c-GPx
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NACRES:
NA.32
UNSPSC Code:
12161503
Service technique
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usage
sufficient for 100 tests
shelf life
Kit is stable for 24 months when unopened.
detection method
colorimetric
shipped in
wet ice
storage temp.
−20°C
General description
The Glutathione Peroxidase Cellular Activity Assay kit is used to measure glutathione peroxidase in tissue extracts. The assay is based on the oxidation of glutathione (GSH) to oxidized glutathione (GSSG). This is catalyzed by GPx coupled to the recycling of GSSG back to GSH utilizing glutathione reductase and NADPH. The decrease in NADPH absorbance measured at 340 nm during the oxidation of NADPH to NADP is indicative of glutathione peroxidase activity since the enzyme is the rate-limiting factor of the coupled reactions.
Application
Suitable for the measureent of glutathione peroxidase in tissue extracts
Biochem/physiol Actions
The Glutathione Peroxidase Cellular Activity Assay kit is used to measure glutathione peroxidase in tissue extracts. The assay is based on the oxidation of glutathione (GSH) to oxidized glutathione (GSSG). This is catalyzed by GPx coupled to the recycling of GSSG back to GSH utilizing glutathione reductase and NADPH. The decrease in NADPH absorbance measured at 340 nm during the oxidation of NADPH to NADP is indicative of glutathione peroxidase activity since the enzyme is the rate-limiting factor of the coupled reactions.
Other Notes
This kit was tested with rabbit reticulocyte lysate and with glutathione peroxidase enzyme.
Composants de kit seuls
Réf. du produit
Description
- tert-Butyl hydroperoxide 1 mL
signalword
Danger
Hazard Classifications
Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Org. Perox. F - Skin Corr. 1C - Skin Sens. 1 - STOT SE 3
target_organs
Respiratory system
Classe de stockage
5.2 - Organic peroxides and self-reacting hazardous materials
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Contenu apparenté
V R Arruda et al.
Neoplasma, 43(2), 99-102 (1996-01-01)
Red cell antioxidant enzymes have been recently studied in malignant lymphomas and the results are controversial. Hairy cell leukemia is a rare chronic lymphoproliferative disorder originating probably in a pluripotent stem cell. In the present study, glutathione peroxidase (Gpx), reduced
International Committee for Standardization in Haematology: recommended methods for red-cell enzyme analysis.
E Beutler et al.
British journal of haematology, 35(2), 331-340 (1977-02-01)
Ian M Scott et al.
Pesticide biochemistry and physiology, 141, 9-17 (2017-09-16)
Plant elicitors can be biological or chemical-derived stimulators of jasmonic acid (JA) or salicylic acid (SA) pathways shown to prime the defenses in many crops. Examples of chemical elicitors of the JA and SA pathways include methyl-jasmonate and 1,2,3-benzothiadiazole-7-carbothioate (BTH
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| CGP1-1KT | 04061833531761 |