MDCK

85011435, dog kidney, Epithelial-like

• Synonyme(s) : MDCK1 Cells, MDCKI Cells, Madin Darby Canine Kidney Cells, NBL-2 Cells
• Code UNSPSC : 41106514
• eCl@ss : 32190102

Propriétés

• Nom du produit: MDCK, 85011435
• Source biologique: dog kidney
• Mode de croissance: Adherent
• Caryotype: 2n = 78
• Morphologie: Epithelial-like
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Canine Cocker Spaniel kidney

Description de la lignée cellulaire

Derived from the kidney of a normal adult Cocker Spaniel in 1958. Useful in viral studies, supports the growth of vesicular exanthema virus of swine, vesicular stomatitis, vaccina, coxsackie B-5, adenoviruses, reoviruses and infectious canine hepatitis. Also known as MDCK (NBL-2).

Application

Virus studies: SVEV, VSV, Vaccinia, reoviruses and inf. canine hepatitis virus

Milieu de culture

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Cells attach firmly and require at least 2 PBS washes prior to addition of trypsin/EDTA.

Autres remarques

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