CAPHOS
Calcium Phosphate Transfection Kit
Most cost effective transfection reagent kit for transient and stable transfection of DNA into mammalian cells
Synonyme(s) :
Gene delivery
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About This Item
Produits recommandés
Qualité
for molecular biology
Niveau de qualité
Forme
solution
Utilisation
kit sufficient for 160 transfections (6 cm dishes)
kit sufficient for 400 transfections (3.5 cm dishes)
kit sufficient for 80 transfections (10 cm dishes)
Technique(s)
transfection: suitable
Conditions d'expédition
dry ice
Température de stockage
−20°C
Description générale
Calcium phosphate transfection is a commonly used method for the introduction of DNA into eukaryotic cells. This technique has been used to obtain both transient and stable transfections in a wide variety of cell types.
Application
Calcium Phosphate Transfection Kit has been used:
- to enable transfection
- to transfect Hek293T cells
- to transfect H29D cells
Suitable for transient and stable transfection of DNA into cultured mammalian cells. The following cells have successfully been transfected using the calcium phosphate method:
BAEC
Bowels melanoma cells
CHO K1
COS-7
Fibroblasts (human embryonic, neo derm)
HEK293
Huh 7
IMR-90
LLC (Lewis Lung Carcinoma)
NIH3T3
PC-12
PCI-13
SH-Sy5Y
SK-Hep-1
T47D
BAEC
Bowels melanoma cells
CHO K1
COS-7
Fibroblasts (human embryonic, neo derm)
HEK293
Huh 7
IMR-90
LLC (Lewis Lung Carcinoma)
NIH3T3
PC-12
PCI-13
SH-Sy5Y
SK-Hep-1
T47D
Caractéristiques et avantages
- Suitable for transient and stable transfection
- Reproducible for a wide range of cell types
- Widely referenced
- Inexpensive
Composants
The Calcium Phosphate Transfection Kit contains:
5 ml 2.5M CaCl2 (C2052)
25 ml 2x HEPES Buffered Saline (H1012)
25 ml molecular biology grade water (W4502)
5 ml 2.5M CaCl2 (C2052)
25 ml 2x HEPES Buffered Saline (H1012)
25 ml molecular biology grade water (W4502)
Principe
The procedure is based on slow mixing of HEPES-buffered saline containing sodium phosphate with a CaCl2 solution containing the DNA. A DNA-calcium phosphate co-precipitate forms, which adheres to the cell surface and is taken up by the cell, presumably by endocytosis.
Autres remarques
The protocol was developed for transient transfection of CHO cells using pSV40-CAT plasmid as a reporter gene.
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2
Code de la classe de stockage
12 - Non Combustible Liquids
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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