C8482
Protein Kinase A catalytic subunit human
>90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution
Synonyme(s) :
Cyclic AMP-dependent protein kinase catalytic subunit, histidine-tagged, recombinant human
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About This Item
Produits recommandés
Produit recombinant
expressed in E. coli
Niveau de qualité
Essai
>90% (SDS-PAGE)
Forme
buffered aqueous glycerol solution
Activité spécifique
>1000 units/mg protein
Poids mol.
43.5 kDa
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Température de stockage
−70°C
Informations sur le gène
human ... PRKACA(5566)
Actions biochimiques/physiologiques
Ubiquitous serine-threonine kinase that phosphorylates a broad spectrum of substrates, and regulates many cellular processes. The catalytic subunit is released following binding of cyclic AMP to the regulatory subunits of the PKA holoenzyme. The free catalytic subunit has intrinsic activity and does not require added cyclic AMP.
Forme physique
Solution in 30 mM potassium phosphate buffer, pH 7.4, containing 50% glycerol, 150 mM KCl, 1 mM EDTA, and 1 mM DTT.
Remarque sur l'analyse
One unit will transfer 1 nanomole of phosphate from 32P-ATP to kemptide substrate per minute at pH 7.4 at 30 °C (measured by a radioactive filter-binding assay).
Km (ATP) = 25 μM at 30 °C, pH 7.4. Km (kemptide) = 42 μM (33 μg/ml) at 30 °C, pH 7.4.
Km (ATP) = 25 μM at 30 °C, pH 7.4. Km (kemptide) = 42 μM (33 μg/ml) at 30 °C, pH 7.4.
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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