C7762
α-Chymotrypsin from bovine pancreas
Type I-S, essentially salt-free, lyophilized powder
Synonyme(s) :
α-chymotrypsin A and B, alpha-chymotrypsin
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About This Item
Produits recommandés
Type
Type I-S
Niveau de qualité
Forme
essentially salt-free, lyophilized powder
Activité spécifique
≥40 units/mg protein
Poids mol.
25 kDa
Produit purifié par
3× crystallization
Solubilité
1 mM HCl: soluble 2.0 mg/mL, clear
Numéro d'accès UniProt
Température de stockage
−20°C
Informations sur le gène
cow ... CTRB1(618826)
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Catégories apparentées
Application
α-Chymotrypsin from bovine pancreas has been used in a study to investigate protein extraction by Winsor-III microemulsion systems. α-Chymotrypsin from bovine pancreas has also been used in a study to investigate a new specific fullerene-based fluorescent probe for trypsin.
The product has been used to investigate the inhibitory effect of several ether oligomers against the enzyme. It has also been used to cleave pro-phenoloxidase in order to estimate total phenoloxidase in haemolymph. Furthermore, the enzyme has been used as a positive control in chymotrypsin assays using salivary gland and anterior midgut extracts of Deraeocoris nigritulus.
Actions biochimiques/physiologiques
α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. The pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca2+ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, and α2-macroglobulin, as well as 10 mM of Cu2+ and Hg2+.
A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond.
Définition de l'unité
One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.
Notes préparatoires
Prepared free of autolysis products and low molecular weight contaminants.
The powder may be reconstituted in 1 mM HCl at 2 mg/mL concentration to form a clear solution.
Remarque sur l'analyse
Minimum 85% protein
Protein determined by E1%/280
Autres remarques
View more information on chymotrypsin at www.sigma-aldrich.com/enzymeexplorer
Inhibiteur
Réf. du produit
Description
Tarif
Substrat
Réf. du produit
Description
Tarif
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Organes cibles
Respiratory system
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Équipement de protection individuelle
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Certificats d'analyse (COA)
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[structure: see text] Hydroxyaromatic aldehydes and ketones were used as building blocks to prepare ether oligomers. An iterative two-step protocol involving Mitsunobu coupling and carbonyl reduction provided a protecting-group-free route with high yields. Activity screening of an 84-member library against
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