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Key Documents

C5338

Sigma-Aldrich

Cyanogen bromide-activated-Sepharose™ 4 Fast Flow

lyophilized powder

Synonyme(s) :

Cyanogen bromide-activated Agarose

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About This Item

Numéro CAS:
68987-32-6
Numéro MDL:
MFCD00163385
Code UNSPSC :
41106500
Nomenclature NACRES :
NA.56

Forme

lyophilized powder

Niveau de qualité

200

Technique(s)

affinity chromatography: suitable

Matrice

Sepharose 4 Fast Flow

Groupe de la matrice active

cyanate or related structures

Espaceur de matrice

1 atom (when ligands are coupled via isourea derivative or related linkage)

Gonflement

1 g swells to 4-5 mL gel

Taille des particules

45—165 μm

Capacité

6-11 μmol/mL coupling capacity (6-AKS)

Température de stockage

2-8°C

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Description générale

C5338-10G′s update product number is GE17-0981-01
Cyanogen bromide-activated-Sepharose® 4 Fast Flow is a resin composed of cross-linked 4% agarose beads that has been pre-activated with cyanogen bromide. Proteins and other molecules containing primary amino groups are coupled directly to the preactivated gel. It allows for multi-point attachment of protein ligands which minimizes ligand leakage. The resin is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.

Application

Cyanogen bromide-activated-Sepharose™ is used in affinity chromatography and protein chromatography. Cyanogen bromide-activated-Sepharose™ has been used to discover insecticidal agents, to raise polyclonal antibodies against the yeast invertase glycosyls, and to study immunological processes of xenograft rejection.

Forme physique

Lyophilized powder with sugar stabilizers

Informations légales

Sepabeads is a registered trademark of Mitsubishi Chemical Corp.
Sepharose is a trademark of Cytiva

Remplacé(e)(s) par

Réf. du produit
Description
Tarif

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Joyce M Bevington et al.
Virology, 357(1), 102-113 (2006-09-09)
Adeno-associated virus (AAV) is a human parvovirus that normally requires a helper virus such as adenovirus (Ad) for replication. The four replication proteins (Rep78, 68, 52 and 40) encoded by AAV are pleiotropic effectors of virus integration, replication, transcription and
M H Zeng et al.
Transplantation proceedings, 38(10), 3312-3314 (2006-12-19)
Human natural killer (NK) cells, which can mediate direct lysis of porcine endothelial cells, play an important role in xenograft rejection. HLA-G, which is a critical molecule in maintaining maternal immune tolerance of semi-allogenic fetus, is able to protect susceptible
F Jafri et al.
Biotechnology and bioengineering, 56(6), 605-609 (2008-07-22)
Polyclonal antibodies directed against the yeast invertase glycosyls were raised by immunizing rabbits with neoglycoprotein-I and neoglycoprotein-II. The neoglycoproteins were prepared by separately coupling the N-linked large and small molecular weight yeast invertase oligosaccharides respectively to bovine serum albumin with
Regina Maria Sousa de Araújo et al.
Plant science : an international journal of experimental plant biology, 183, 20-26 (2011-12-27)
Hemagglutinating activity has been associated to presence of lectin, carbohydrate-binding proteins. In this work Crataeva tapia bark lectin (CrataBL) was purified in milligram quantities (28 mg per g of bark) by ion exchange chromatography. The lectin was thermo-stable, ion-independent and

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