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C4018

Sigma-Aldrich

Choloylglycine Hydrolase from Clostridium perfringens (C. welchii)

lyophilized powder, ≥100 units/mg protein

Synonyme(s) :

3α,7α,12α-Trihydroxy-5β-cholan-24-oylglycine amidohydrolase

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About This Item

Numéro CAS:
37289-07-9
Numéro de classification (Commission des enzymes):
3.5.1.24 (BRENDA, IUBMB)
Numéro MDL:
MFCD00130789
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Forme

lyophilized powder

Niveau de qualité

200

Activité spécifique

≥100 units/mg protein

Composition

Protein, ≥50% biuret

Conditions d'expédition

wet ice

Température de stockage

−20°C

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Application

The enzyme from Sigma has been used in the analysis of bile samples in various studies.

Actions biochimiques/physiologiques

The enzyme is an N-terminal nucleophilic (Ntn) hydrolase that catalyzes the hydrolysis of amide bonds, liberates the glycine/taurine moiety from the steroid core and eventually yields unconjugated bile acids. Agents that oxidize thiol groups (e.g., p-mercuribenzoate, iodoacetamide, Hg2+, Cu2+, and Cd2+) have been shown to strongly inhibit bile salt hydrolase (BSH) activity in Clostridium perfringens.

Définition de l'unité

One unit will hydrolyze 1.0 μmole of glycocholic acid to glycine and cholic acid in 5 min at pH 5.6 at 37 °C.

Forme physique

Partially purified lyophilized powder containing buffer salts and stabilizer

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H334

Conseils de prudence

P261 - P284 - P501

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
0000282850
0000142044
0000188551
0000282850
0000188551

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Maksim Rossocha et al.
Biochemistry, 44(15), 5739-5748 (2005-04-13)
Bacterial bile salt hydrolases catalyze the degradation of conjugated bile acids in the mammalian gut. The crystal structures of conjugated bile acid hydrolase (CBAH) from Clostridium perfringens as apoenzyme and in complex with taurodeoxycholate that was hydrolyzed to the reaction
M Victoria Delpino et al.
Archives of microbiology, 191(7), 571-581 (2009-05-14)
The virB genes coding type IV secretion system are necessary for the intracellular survival and replication of Brucella spp. In this study, extracellular proteins from B. abortus 2308 (wild type, WT) and its isogenic virB10 polar mutant were compared. Culture
Christopher Martoni et al.
Applied microbiology and biotechnology, 81(2), 225-233 (2008-08-23)
This is the first study of its kind to screen probiotic lactic acid bacteria for the purpose of microencapsulating a highly bile salt hydrolase (BSH)-active strain. A Lactobacillus reuteri strain and a Bifidobacterium longum strain were isolated as the highest
Genta Kakiyama et al.
Journal of lipid research, 61(12), 1629-1644 (2020-10-04)
NAFLD is an important public health issue closely associated with the pervasive epidemics of diabetes and obesity. Yet, despite NAFLD being among the most common of chronic liver diseases, the biological factors responsible for its transition from benign nonalcoholic fatty
Arekal N Roopashri et al.
Applied microbiology and biotechnology, 83(6), 1115-1126 (2009-05-02)
In a changing scenario of food habits being associated with wellness factors through the concepts of probiotics and prebiotics, an attempt has been made to characterize on molecular basis, the desirable benefits associated with natural isolates of lactic acid bacteria
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