C0775

Crystal violet

Dye content, ≥90%, certified by the Biological Stain Commission, powder

• Synonyme(s) : Chlorure d’hexaméthylpararosaniline, Violet 3 de base, Violet de gentiane, Violet de méthyle 10B
• Formule linéaire : C₂₅H₃₀N₃Cl
• Numéro CAS: 548-62-9
• Poids moléculaire : 407.98
• Numéro C.I. (Colour Index): 42555
• Numéro Beilstein : 3580948
• Numéro CE : 208-953-6
• Numéro MDL: MFCD00011750
• Code UNSPSC : 12171500
• ID de substance PubChem : 57653980
• Nomenclature NACRES : NA.47

Propriétés

• product name: Crystal violet, certified by the Biological Stain Commission
• Qualité: certified by the Biological Stain Commission
• Niveau de qualité: 200
• Forme: powder
• Composition: Dye content, ≥90%
• Technique(s): microbe id | staining: suitable
• Couleur: green to very dark green
• pH: 2.5 — 3.5 (20 °C)
• Pf: 205 °C (dec.) (lit.)
• Solubilité: H₂O: 1 mg/mL
• Densité: 1.19 g/cm³ at 20 °C
• Spectre d'activité de l'antibiotique: fungi
• Application(s): diagnostic assay manufacturing; hematology; histology
• Mode d’action: cell membrane | interferes; enzyme | inhibits
• Température de stockage: room temp
• Chaîne SMILES: [Cl-].CN(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](/C)C
• InChI: 1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1
• Clé InChI: ZXJXZNDDNMQXFV-UHFFFAOYSA-M

Description

Application

Crystal violet is used as an active component, primary stain, of Gram stain for differentiation of Gram-negative versus Gram-positive bacteria.
It has been used to check cell viability. It has also been used for the staining of cells to study cell migration and invasion.

Actions biochimiques/physiologiques

Crystal violet can be used for DNA visualization in agarose gels. The dye is used only in the presence of high concentrations of DNA. Crystal violet is also used for the staining of bacteria in gram staining technique. It is also used for the staining of plant chromosomes. Crystal Violet also helps in colorimetric measurement of cell viability.

Adéquation

Certified for use with the Gram stain for bacteria, Flemming triple stain with iodine for chromosomes, Lieb′s amyloid stain and Newton′s crystal violet-iodine technique for chromatin and nucleoli.

Informations sur la sécurité

• Pictogrammes: GHS08,GHS05,GHS07,GHS09
• Mention d'avertissement: Danger
• Mentions de danger: H302,H318,H351,H410
• Conseils de prudence: P202 - P273 - P280 - P301 + P312 - P305 + P351 + P338 - P308 + P313
• Classification des risques: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable