BCK-EDU594
EdU-Click 594
sufficient for 100 assays
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About This Item
Produits recommandés
Fluorescence
λex 584 nm; λem 603 nm
Conditions d'expédition
wet ice
Température de stockage
2-8°C
Description générale
EdU (5-ethynyl-2′-deoxyuridine) is a nucleoside analog to thymidine and is incorporated into DNA during active DNA synthesis. In contrast to BrdU assays, the EdU-Click Assays are not antibody based and therefore do not require DNA denaturation for detection of the incorporated nucleoside. Instead, the Baseclick EdU-Click Assays utilize click chemistry for detection in a variety of dye fluorescent readouts. Furthermore, the streamlined detection protocol reduces both the total number of steps and significantly decreases the total amount of time. The simple click chemistry detection procedure is complete within 30 minutes and is compatible with multiplexing for content and context-rich results.
Application
Imaging
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Aquatic Chronic 3 - Eye Irrit. 2 - Muta. 1B - Repr. 2
Code de la classe de stockage
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
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Les clients ont également consulté
Journal of immunological methods, 350(1-2), 29-35 (2009-08-04)
T lymphocyte proliferations can be measured by [(3)H]thymidine incorporation. However, many labs avoid this technique because of the need to use radioactive substrates. In addition, [(3)H]thymidine incorporation method does not permit simultaneous characterization of the proliferating cells. We developed the
Journal of neuroscience methods, 177(1), 122-130 (2008-11-11)
Labelling and identifying proliferating cells is central to understanding neurogenesis and neural lineages in vivo and in vitro. We present here a novel thymidine analogue, ethynyl deoxyuridine (EdU) for labelling dividing cells, detected with a fluorescent azide which forms a
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
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