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Merck
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Principaux documents

B8520

Sigma-Aldrich

Anti-Mouse IgG (whole molecule)−Biotin antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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About This Item

Numéro MDL:
MFCD00162641
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

rabbit

Niveau de qualité

200

Conjugué

biotin conjugate

Forme d'anticorps

IgG fraction of antiserum

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Technique(s)

direct ELISA: 1:200,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:300
western blot: 1:400,000-1:800,000

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Description générale

Immunoglobulins (Igs) belongs to the immunoglobulin super-family. There are five different types of Immunoglobulins. Each immunoglobulin has two heavy (H) and two light (L) chains held together by disulfide linkages. Each light comprises of one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region. IgG is abundant immunoglobulin in human serum.

Immunogène

Purified mouse IgG

Application

Anti-Mouse IgG (whole molecule)-Biotin antibody has been used in
  • immunofluorescence
  • immunoblot
  • direct enzyme linked immunosorbent assay (ELISA)
  • serotyping assay
  • immunohistochemistry

Actions biochimiques/physiologiques

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.

Forme physique

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Muna F Anjum et al.
Clinical and vaccine immunology : CVI, 13(5), 561-567 (2006-05-10)
Serological typing of Escherichia coli O antigens is a well-established method used for differentiation and identification of O serotypes commonly associated with disease. In this feasibility study, we have developed a novel somatic antibody-based miniaturized microarray chip, using 17 antisera
Structural and functional composition of the developing retinogeniculate pathway in the mouse
Jaubert-miazza L, et al.
Visual Neuroscience, 22, 661-676 (2005)
In Vitro and In Vivo Detection of Mx Gene Products in Bovine Cells following Stimulation with α/β Interferon and Viruses
Muller-Doblies, D., et al.
Clinical and Vaccine Immunology : CVI, 9(6), 1192-1199 (2002)
Ming-Shu Hsieh et al.
NPJ vaccines, 8(1), 82-82 (2023-06-03)
Formyl peptide receptor-like 1 inhibitor protein (FLIPr) is an immune evasion protein produced by Staphylococcus aureus, and FLIPr is a potential vaccine candidate for reducing Staphylococcus aureus virulence and biofilm formation. We produced recombinant lipidated FLIPr (rLF) to increase the
E P S Conceição et al.
The Journal of endocrinology, 230(2), 263-274 (2016-06-22)
Rats overfed during lactation show higher visceral adipose tissue (VAT) mass and metabolic dysfunctions at adulthood. As both vitamin D and glucocorticoids change adipogenesis, parameters related to metabolism and action of these hormones in the adipocyte can be altered in
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