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Merck
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Principaux documents

B5926

Sigma-Aldrich

10× REDTaq® PCR Reaction Buffer

To be used with REDTaq® DNA Polymerase

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About This Item

Code UNSPSC :
41106306
Nomenclature NACRES :
NA.52

Niveau de qualité

Forme

liquid

Couleur

colorless

Température de stockage

−20°C

Description générale

10x REDTaq® PCR Reaction Buffer is a polymerase chain reaction buffer for use with REDTaq® DNA Polymerase (D4309).

Application

10× REDTaq® PCR Reaction Buffer has been used as a component of reaction mix:

  • for PCR detection of virulence genes from Campylobacter jejuni food and clinical isolates in BALB/c mice
  • for PCR detection of virulence genes of Campylobacter coli isolates from infected mice liver
  • for identifying K-ras mutated alleles by PCR-restriction fragment length polymorphism (RFLP) in patients with colorectal carcinoma

Informations légales

REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Consulter la Bibliothèque de documents

Christoph P Dieterle et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 10(2), 641-650 (2004-02-05)
The aim of this study was to identify K-ras mutations as marker for isolated tumor cells in liver, lymph node, and bone marrow specimens of colorectal cancer patients. To detect these, a PCR-RFLP assay was used with a sensitivity exceeding
Birte Meyer et al.
Microbiology (Reading, England), 153(Pt 7), 2026-2044 (2007-06-30)
Newly developed PCR assays were used to PCR-amplify and sequence fragments of the dissimilatory adenosine-5'-phosphosulfate (APS) reductase genes (aprBA) comprising nearly the entire gene locus (2.2-2.4 kb, equal to 92-94 % of the protein coding sequence) from 75 sulfate-reducing prokaryotes
Virulence comparison of human and poultry campylobacter Jejuni isolates in a mouse model
Vuvckovic D, et al.
Medical Research Engineering, 5(10) (2017)
Romain Marti et al.
Applied and environmental microbiology, 75(15), 4967-4974 (2009-06-16)
The objective of this study was to identify a microbial marker for pig manure contamination. We quantified the persistence of four dominant bacterial groups from the pig intestinal tract throughout manure handling at 10 livestock operations (including aerobic digestion) by
Chris Waskewich et al.
Cancer research, 62(7), 2029-2033 (2002-04-04)
Cyclooxygenase-2 (COX-2) is an important cellular target for both therapy and/or prevention of inflammatory disorders and cancer. The advent of selective COX-2 inhibitors now allows a more precise and safer treatment approach. The screening of an array of cancer cell

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