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Merck
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Principaux documents

B3804

Sigma-Aldrich

BCIP®/NBT-Blue

alkaline phosphatase substrate, chromogenic, liquid

Synonyme(s) :

5-Bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium liquid substrate system

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About This Item

Code UNSPSC :
12352204
Nomenclature NACRES :
NA.83

product name

BCIP®/NBT-Blue Liquid Substrate System for Membranes, alkaline phosphatase substrate

Niveau de qualité

Forme

liquid

Température de stockage

2-8°C

Description générale

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-bromo4-chloro-3-indolyl phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end-product that is blue to purple and can be observed visually. BCIP®/NBT-blue liquid substrate system for membranes is a specifically formulated liquid enzyme substrate that is stable and optimized for blotting applications offers maximum convenience, time savings, and consistency.

Application

BCIP®/NBT-Blue Liquid Substrate System for Membranes has been used in colorimetric detection of alkaline phosphatase (ALP) activity such as western blot analysis.

Attention

Stable at least one year at 2-8 °C. This substrate is light-sensitive.

Autres remarques

Not recommended for microwell (e.g., ELISA) or immunohistochemical applications.

Informations légales

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Stephen P Christiansen et al.
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