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A8667

Sigma-Aldrich

Anti-Human IgG (whole molecule)−Peroxidase antibody produced in goat

IgG fraction of antiserum, buffered aqueous solution

Synonyme(s) :

Goat Anti-Human IgG (whole molecule) HRP

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About This Item

Numéro MDL:
MFCD00162456
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

goat

Niveau de qualité

300

Conjugué

peroxidase conjugate

Forme d'anticorps

IgG fraction of antiserum

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Espèces réactives

human

Technique(s)

direct ELISA: 1:30,000
dot blot: 1:80,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Description générale

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders.
Goat Anti-Human IgG (whole molecule)-Peroxidase antibody binds to human IgG.

Immunogène

human IgG

Application

Anti-Human IgG (whole molecule)-Peroxidase antibody produced in goat has also been used as a conjugate for gold nanoparticles (AuNPs) in electrochemical detection.
Goat Anti-Human IgG (whole molecule)-Peroxidase antibody has been used for ELISA, dot blot and immunohistochemistry.
Specificities of anti-human HIV-1 envelope antibodies was determined by ELISA using HRP-conjugated goat anti-human IgG at a dilution of 1:1000.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT

Notes préparatoires

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H317,H334

Classification des risques

Resp. Sens. 1 - Skin Sens. 1

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Cairen Chen et al.
FEBS open bio, 6(3), 211-215 (2016-04-06)
A recent study reported that circulating antibodies to CD25-derived peptide antigens were significantly higher in patients with nonsmall cell lung cancer (NSCLC) than control subjects. The present study was, thus, undertaken to replicate the initial finding with different sample sets.
Xueying Zhang et al.
FEBS open bio, 5, 712-716 (2015-10-02)
A number of studies have reported an association between increased levels of antibodies against oxidized low-density lipoprotein (oxLDL) and cardiovascular disease, but the anti-oxLDL antibody has not been confirmed to serve as an effective biomarker for prediction of acute myocardial
Controlling the electrochemical deposition of silver onto gold nanoparticles: Reducing interferences and increasing the sensitivity of magnetoimmuno assays
de la EM, et al.
Biosensors And Bioelectronics, 24(8), 2475-2482 (2009)
Leiguang Ye et al.
FEBS open bio, 5, 809-812 (2015-11-14)
An in-house enzyme-linked immunosorbent assay (ELISA) was developed in this study to detect circulating IgG antibodies to peptide antigens derived from baculoviral IAP repeat-containing protein 5 isoform 2 (BIRC5) and myc proto-oncogene protein (MYC) in non-small cell lung cancer (NSCLC).
Marzieh Ashrafmansouri et al.
Iranian journal of parasitology, 10(4), 599-604 (2016-01-27)
Cutaneous leishmaniasis (CL) is a parasitic disease with a relatively wide distribution in different areas of the world, including Iran. The parasite is mainly diagnosed microscopically, but serological approaches might be useful for diagnosis as well. This study aimed to
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