A3678

Ammonium persulfate

for molecular biology, suitable for electrophoresis, ≥98%

• Synonyme(s) : AP, APS, Ammonium peroxodisulfate, Ammonium peroxydisulfate, PER
• Formule linéaire : (NH₄)₂S₂O₈
• Numéro CAS: 7727-54-0
• Poids moléculaire : 228.20
• Numéro CE : 231-786-5
• Numéro MDL: MFCD00003390
• Code UNSPSC : 41105319
• eCl@ss : 38030421
• ID de substance PubChem : 24890800
• Nomenclature NACRES : NB.22

Propriétés

• Qualité: for molecular biology
• Niveau de qualité: 200
• Densité de vapeur: 7.9 (vs air)
• Pureté: ≥98%
• Forme: powder
• Pertinence de la réaction: reagent type: oxidant
• Technique(s): electrophoresis: suitable
• Traces d'anions: chloride (Cl^-): <10 ppm
• Traces de cations: Fe: <10 ppm; heavy metals (as Pb): <50 ppm
• Activité étrangère: DNAse, none detected; Protease, none detected; RNAse, none detected
• Chaîne SMILES: N.N.OS(=O)(=O)OOS(O)(=O)=O
• InChI: 1S/2H3N.H2O8S2/c;;1-9(2,3)7-8-10(4,5)6/h2*1H3;(H,1,2,3)(H,4,5,6)
• Clé InChI: ROOXNKNUYICQNP-UHFFFAOYSA-N

Description

Description générale

Ammonium persulfate (APS) is a widely used reagent in biochemistry and molecular biology for the preparation of polyacrylamide gels. APS forms oxygen free radicals in aqueous solution by a base-catalyzed mechanism. The bases, most commonly used as catalysts, are tertiary amines such as TEMED (N,N,N′,N′-tetramethylethylenediamine) or DMAPN (3-dimethylaminopropionitrile). The free radicals will cause the polymerization of acrylamide and bis-acrylamide to form a gel matrix, which can be used for separating macromolecules by size. APS has also been utilized to study protein-protein interactions via photoinitiated crosslinking chemistry.

Application

Ammonium persulfate has been used for the preparation of polyacrylamide gels and acrylamide hydrogels.

Catalyst for acrylamide gel polymerization.

Remarque sur l'analyse

Tested for use in acrylamide polymerization.

Informations sur la sécurité

• Pictogrammes: GHS03,GHS08,GHS07
• Mention d'avertissement: Danger
• Mentions de danger: H272,H302,H315,H317,H319,H334,H335
• Conseils de prudence: P210 - P220 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Classification des risques: Acute Tox. 4 Oral - Eye Irrit. 2 - Ox. Sol. 3 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 5.1B - Oxidizing hazardous materials
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges