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A1595

Sigma-Aldrich

Albumine de sérum bovin solution

10% in DPBS, low endotoxin, fatty acid free, suitable for cell culture, sterile-filtered

Synonyme(s) :

Albumine solution from bovine serum, Solution de BSA

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About This Item

Numéro CAS:
9048-46-8
Numéro MDL:
MFCD00130384
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.27

Source biologique

bovine

Niveau de qualité

300

Stérilité

sterile-filtered

Gamme de produits

BioReagent

Pureté

90-120 mg/mL protein basis (biuret)

Forme

solution

Produit purifié par

heat shock fractionation

Contient

does not contain stabilizer or preservative

Origine

USA origin

Concentration

10% in DPBS

Technique(s)

cell culture | mammalian: suitable

Impuretés

≤0.05% fatty acid
≤200 EU/mL endotoxin

pH

7.1-7.7

Numéro d'accès UniProt

P02769

Température de stockage

2-8°C

Informations sur le gène

bovine ... ALB(280717)

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Catégories apparentées

Application

Recommended for use as a base for the preparation of defined, fatty acid supplements. Can be loaded with up to 4 moles of fatty acids per mole of albumin.

BSA is a known fatty acid (FA) carrier. Thus in FA-related applications like cell culture, the specific BSA can be important, as related to control over specific fatty acids used to culture cell lines, because different cell lines can be variably sensitive to particular FA;s. Fatty acid-free BSA therefore is useful in cell culture work where the specific fatty acid content must be carefully controlled, so that researchers can control the specific FA′s needed for their particular cell lines. Fatty acid-free BSA also allows for optimal and maximum binding sites for the specific fatty acids that researchers wish to use in their particular cell culture experiments. Use of fatty-acid-free BSA also addresses concerns about endogenous FA′s potentially in non-fatty acid-free BSA.

Caractéristiques et avantages

  • Fatty acid free / tested to be low in fatty acid content
  • Low endotoxin
  • Sterile-filtered

Notes préparatoires

Prepared with Heat Shock Fractionated fatty acid-free albumin in DPBS D5652.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

08168

Timestrip Plus™ 8 °C

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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Ana Janic et al.
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The Analyst, 145(23), 7680-7686 (2020-09-26)
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Véronique Tuloup-Minguez et al.
Autophagy, 7(1), 27-39 (2010-10-29)
Macroautophagy is a major lysosomal degradation pathway for cellular components in eukaryotic cells. Baseline macroautophagy is important for quality control of the cytoplasm in order to avoid the accumulation of cytotoxic products. Its stimulation by various stressful situations, including nutrient
Guangfeng Geng et al.
eLife, 10 (2021-05-05)
Erythropoietin (EPO) drives erythropoiesis and is secreted mainly by the kidney upon hypoxic or anemic stress. The paucity of EPO production in renal EPO-producing cells (REPs) causes renal anemia, one of the most common complications of chronic nephropathies. Although mitochondrial
Matthieu Dos Santos et al.
STAR protocols, 2(3), 100694-100694 (2021-08-13)
Single-nucleus RNA sequencing allows the profiling of gene expression in isolated nuclei. Here, we describe a step-by-step protocol optimized for adult mouse skeletal muscles. This protocol provides two main advantages compared to the widely used single-cell protocol. First, it allows
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