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86R

Sigma-Aldrich

Leukocyte Alkaline Phosphatase Kit

based on naphthol AS-BI and fast red violet LB

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About This Item

Code UNSPSC :
12352106
eCl@ss :
42010105
Nomenclature NACRES :
NA.47

Niveau de qualité

500

Durée de conservation

Expiry date on the label.

IVD

for in vitro diagnostic use

Application(s)

hematology
histology

Conditions d'expédition

wet ice

Température de stockage

2-8°C

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Application

Leukocyte Alkaline Phosphatase (LAP) kits are intended for the qualitative demonstration of alkaline phosphatase activity in white blood cells.
Peripheral blood or bone marrow preparations are fixed to a microscope slide. The film is then incubated in a mixture of naphthol AS-BI alkaline solution with fast red violet LB. The resulting insoluble diffuse, red dye deposit indicates sites of alkaline phosphatase activity.

Composants de kit seuls

Réf. du produit
Description
  • Citrate Solution (915) 50 mL
  • FRV-Alkaline Solution (862) 10 mL
  • Hematoxylin Solution, Gill No. 3 (kit only) 50 mL
  • Naphthol AS-BI Alkaline Solution (861) 10 mL
  • Sodium Nitrite Solution (914) 10 mL

Pictogrammes

Health hazardCorrosionExclamation mark
GHS08,GHS05,GHS07

Mention d'avertissement

Danger

Mentions de danger

H290,H302,H315,H318,H373

Classification des risques

Acute Tox. 4 Oral - Eye Dam. 1 - Met. Corr. 1 - Skin Irrit. 2 - STOT RE 2 Oral

Organes cibles

Kidney

Code de la classe de stockage

8A - Combustible corrosive hazardous materials

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Edgardo Rodríguez-Carballo et al.
PloS one, 9(7), e102032-e102032 (2014-07-10)
p38 MAPK activity plays an important role in several steps of the osteoblast lineage progression through activation of osteoblast-specific transcription factors and it is also essential for the acquisition of the osteoblast phenotype in early development. Although reports indicate p38
Raha Favaedi et al.
The International journal of developmental biology, 60(4-6), 103-110 (2016-07-09)
Histone H3 lysine 9 methylation has been shown to be a critical barrier to efficient cell reprogramming. This discovery allows the assessment of the cell pluripotency state by considering the extent of H3K9 methylation vs. acetylation at the same position.
Alessandro Polini et al.
PloS one, 6(10), e26211-e26211 (2011-10-25)
The development of a new family of implantable bioinspired materials is a focal point of bone tissue engineering. Implant surfaces that better mimic the natural bone extracellular matrix, a naturally nano-composite tissue, can stimulate stem cell differentiation towards osteogenic lineages
Clive H Glover et al.
PLoS computational biology, 2(11), e158-e158 (2006-11-24)
Stem cell differentiation involves critical changes in gene expression. Identification of these should provide endpoints useful for optimizing stem cell propagation as well as potential clues about mechanisms governing stem cell maintenance. Here we describe the results of a new
Terence P Gade et al.
PloS one, 6(7), e22608-e22608 (2011-07-30)
The purpose of this study was to develop a paradigm for quantitative molecular imaging of bone cell activity. We hypothesized the feasibility of non-invasive imaging of the osteoblast enzyme alkaline phosphatase (ALP) using a small imaging molecule in combination with
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