83702

Rhodamine 123

BioReagent, for fluorescence, ≥85% (HPLC)

• Synonyme(s) : 2-(6-Amino-3-imino-3H-xanthen-9-yl)benzoic acid methyl ester
• Formule linéaire : C₂₁H₁₇ClN₂O₃
• Numéro CAS: 62669-70-9
• Poids moléculaire : 380.82
• Numéro Beilstein : 6030951
• Numéro CE : 263-687-8
• Numéro MDL: MFCD00012664
• Code UNSPSC : 12352116
• ID de substance PubChem : 57652966
• Nomenclature NACRES : NA.32

Propriétés

• Qualité: for fluorescence
• Niveau de qualité: 100
• Gamme de produits: BioReagent
• Pureté: ≥85% (HPLC)
• Forme: powder
• Impuretés: ≤10% water
• Solubilité: ethanol: 20 mg/mL, clear, red
• Adéquation: in accordance for fluorescence
• Chaîne SMILES: Cl[H].COC(=O)c1ccccc1C2=C3C=CC(=N)C=C3Oc4cc(N)ccc24
• InChI: 1S/C21H16N2O3.ClH/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20;/h2-11,22H,23H2,1H3;1H
• Clé InChI: MYFATKRONKHHQL-UHFFFAOYSA-N

Description

Description générale

Rhodamine 123 is a fluorescent dye.

Application

Rhodamine 123 has been used:

• as a substrate for P-glycoprotein (P-gp) to read out functional transporter activity in fluorescent accumulation assays
• to label the cells for estimating the P-gp expression by flow cytometry
• in transport studies, was added to the apical to basolateral (AB-transport) or basolateral to apical (BA-transport)
• the detection of mitochondrial membrane potential (ΔΨm)

Actions biochimiques/physiologiques

Rhodamine 123 is usually used as a functional reporter for P-glycoprotein (P-gp) in flow cytometry.

Fluorescent dye most commonly used in flow cytometry as functional reporter for Pgp. Functional assays for MDR proteins are better prognostic indicators in cancer therapy than levels of MDR protein expression. Rh 123 can be used in multiparameter analyses without fluorescence interference in combination with common protein labeling dyes such as PE-Cy5 and AMCA (7-amino-4-methylcoumarin-3-acetic acid). Recent reports indicate Rh 123 may also be a substrate of MRP1.

Autres remarques

Supravital mitochondrial-specific dye

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)