690PB-100A
Human Mononuclear Cells: HMNC, adult
Synonyme(s) :
HMNC, HMNC cells
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About This Item
Code UNSPSC :
41106514
Nomenclature NACRES :
NA.81
Produits recommandés
Source biologique
human peripheral blood
Forme
solid
Conditionnement
pkg of 10,000,000 cells (HMNC-PB)
Fabricant/nom de marque
Cell Applications, Inc
Mode de croissance
Suspension
Caryotype
2n = 46
Morphologie
mononuclear
Technique(s)
cell culture | mammalian: suitable
Maladie(s) pertinente(s)
acquired immunodeficiency syndrome/human immunodeficiency virus (AIDS/HIV); infectious diseases; autoimmune diseases
Conditions d'expédition
dry ice
Température de stockage
−196°C
Description générale
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The leukocytes can be divided into two groups: mononuclear and granulocytic cells. The mononuclear cells mainly consist of lymphocytes and monocytes. The lymphocyte population consists of T cells (CD4 and CD8 positive ~75%), B cells and NK cells (~25% combined). In contrast to the granulocytes, these cells have rounded nuclei, some with indentations or folds. The mononuclear cells are involved in various ways with the body′s defense mechanisms.
1. These cells are critical components in the immune system to fight bacterial, viral infections, and function as immune surveillance network to kill tumor cells. The mononuclear cells also secrete a great variety of cytokines to regulate immune response as well as inflammation. Moreover, it has been demonstrated that the mononuclear cells may mediate various biological effects on other tissue and organs. For example, the chemokines produced by monocytes can act on adipocytes and muscles. Human Mononuclear Cells (HMNC) are often extracted from from bone marrow (BM) or peripheral blood (PB) using Ficoll-Hypaque density gradient centrifugation, a hydrophilic polysaccharide that separates layers of blood, with mononuclear cells forming a buffy coat under a layer of plasma. Generally, this buffy coat mainly contains lymphocytes and monocytes, other cell types such as cells are detected in Human Mononuclear Cell (HMNC) preparation.
2. Human Mononuclear Cells (HMNC) are widely used in research and clinical applications every day. The HMNCs are important tool for various studies in the fields of immunology (including auto-immune disorders), infectious disease, hematological malignancies, vaccine development, transplant immunology, and high-throughput screening.
3. HIV research uses them because PBMCs include CD4+ cells, which are the cells HIV infects.
4. Moreover, HMNCs have been accepted as a unique material for cancer immunotheurapy using dendritic cells or activated lymphocytes as an alternative or adjuvant to conventional therapies such as surgery, chemotherapy and radiation treatment.
5,6. Endothelial progenitor cells derived from HMNC have been used in neovascularization to improve limb ischemia
7. In cell therapy to repair ischemic and infracted myocardium.
8. Human Mononuclear Cells (HMNC) should provide a useful tool for studying various aspects of pathology and biology of the human mononuclear cells in vitro.
Human Mononuclear Cells (HMNC) are derived from human peripheral blood (HMNC-PB) using Ficoll-Hypaque density gradient centrifugation. They are cryopreserved immediately after isolation.
1. Allison, A.C. et al, Annu. Rev. Immunol. 1:361 (1983); 2. Sales, V.L. et al, Tissue Eng. 13:525 (2007); 3. Juranic, Z. et al, J. Exp. Clin. Cancer Res. 18:317 (1999); 4. Vicenzi E. et al, J. Leukoc. Biol. 68:405 (2000); 5. Steinman, R. M. et al, Int. J. Cancer 94:459 (2001); 6. Schuler, G., Cancer Immunity 3 (Suppl 1):23 (2003); 7. Kalka, C. et al, Proc. Natl. Acad. Sci. USA 97:3422 (2000); 8. Masuda, H. et al, Cardiovasc. Res. 58:390 (2003)
The leukocytes can be divided into two groups: mononuclear and granulocytic cells. The mononuclear cells mainly consist of lymphocytes and monocytes. The lymphocyte population consists of T cells (CD4 and CD8 positive ~75%), B cells and NK cells (~25% combined). In contrast to the granulocytes, these cells have rounded nuclei, some with indentations or folds. The mononuclear cells are involved in various ways with the body′s defense mechanisms.
1. These cells are critical components in the immune system to fight bacterial, viral infections, and function as immune surveillance network to kill tumor cells. The mononuclear cells also secrete a great variety of cytokines to regulate immune response as well as inflammation. Moreover, it has been demonstrated that the mononuclear cells may mediate various biological effects on other tissue and organs. For example, the chemokines produced by monocytes can act on adipocytes and muscles. Human Mononuclear Cells (HMNC) are often extracted from from bone marrow (BM) or peripheral blood (PB) using Ficoll-Hypaque density gradient centrifugation, a hydrophilic polysaccharide that separates layers of blood, with mononuclear cells forming a buffy coat under a layer of plasma. Generally, this buffy coat mainly contains lymphocytes and monocytes, other cell types such as cells are detected in Human Mononuclear Cell (HMNC) preparation.
2. Human Mononuclear Cells (HMNC) are widely used in research and clinical applications every day. The HMNCs are important tool for various studies in the fields of immunology (including auto-immune disorders), infectious disease, hematological malignancies, vaccine development, transplant immunology, and high-throughput screening.
3. HIV research uses them because PBMCs include CD4+ cells, which are the cells HIV infects.
4. Moreover, HMNCs have been accepted as a unique material for cancer immunotheurapy using dendritic cells or activated lymphocytes as an alternative or adjuvant to conventional therapies such as surgery, chemotherapy and radiation treatment.
5,6. Endothelial progenitor cells derived from HMNC have been used in neovascularization to improve limb ischemia
7. In cell therapy to repair ischemic and infracted myocardium.
8. Human Mononuclear Cells (HMNC) should provide a useful tool for studying various aspects of pathology and biology of the human mononuclear cells in vitro.
Human Mononuclear Cells (HMNC) are derived from human peripheral blood (HMNC-PB) using Ficoll-Hypaque density gradient centrifugation. They are cryopreserved immediately after isolation.
1. Allison, A.C. et al, Annu. Rev. Immunol. 1:361 (1983); 2. Sales, V.L. et al, Tissue Eng. 13:525 (2007); 3. Juranic, Z. et al, J. Exp. Clin. Cancer Res. 18:317 (1999); 4. Vicenzi E. et al, J. Leukoc. Biol. 68:405 (2000); 5. Steinman, R. M. et al, Int. J. Cancer 94:459 (2001); 6. Schuler, G., Cancer Immunity 3 (Suppl 1):23 (2003); 7. Kalka, C. et al, Proc. Natl. Acad. Sci. USA 97:3422 (2000); 8. Masuda, H. et al, Cardiovasc. Res. 58:390 (2003)
Origine de la lignée cellulaire
Human Peripheral Blood
Application
immune surveillance and defense, cytokine and chemokine production, immunoregulation, research, clinical applications, vaccine development, transplant immunology, high-throughput screening, HIV research, cancer immunotherapy, infection fighting, study of T, B and NK cells,
Composants
HMNC-PB in DMEM containing 45% FBS & 10% DMSO
Notes préparatoires
- Immediately after isolation, 10,000,000 HMNC-PB in DMEM containing 45% FBS & 10% DMSO
- Blood cells do not proliferate in culture
Procédure de repiquage
Please refer to the HMNC-PB Culture Protocol.
Clause de non-responsabilité
Ce produit, destiné à la recherche scientifique, est soumis à une réglementation spécifique en France, y compris pour les activités d'importation et d'exportation (Article L 1211-1 alinéa 2 du Code de la Santé Publique). L'acheteur (c'est-à-dire l'utilisateur final) est tenu d'obtenir une autorisation d'importation auprès du Ministère français de la Recherche, mentionné à l'article L1245-5-1 II du Code de la Santé Publique. En commandant ce produit, vous confirmez détenir l'autorisation d'importation requise.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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