51412C

Milieu essentiel minimum d′Eagle

with Earle′s Balanced Salts, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

• Synonyme(s) : MEM
• Code UNSPSC : 12352207
• Nomenclature NACRES : NA.75

Propriétés

• Description: for research or for further manufacturing use
• Niveau de qualité: 500
• Stérilité: sterile-filtered
• Forme: liquid
• Technique(s): cell culture | mammalian: suitable; cell culture | stem cell: suitable
• Composants: L-glutamine: no; NaHCO₃: 2200 mg/L; phenol red: 10.62 mg/L; Earle’s salts (5% CO₂): yes
• Température de stockage: 2-8°C

Description

Description générale

Minimum Essential Medium (MEM) with Earle′s Balanced Salts was developed by Harry Eagle as a result of his studies to determine the essential nutritional requirements of mouse L cells and HeLa cells in culture. He demonstrated that these cells require 13 amino acids and seven vitamins to grow and reproduce in vitro. MEM is a modification of Eagle′s earlier medium Basal Medium Eagle (BME), containing higher concentrations of the essential nutrients. This medium has demonstrated the ability to support a variety of normal and transformed cells in culture and contains Earle′s Balanced Salts, which makes it suitable for use in atmospheres charged with CO₂ gas.

Application

Minimum essential medium (MEM) has been used as a growth medium for bone marrow mesenchymal stem cells (BMSCs) and neurons.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves