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46950

Sigma-Aldrich

Fluorescein 5(6)-isothiocyanate

BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)

Synonyme(s) :

FITC, Fluorescein isothiocyanate

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About This Item

Formule empirique (notation de Hill):
C21H11NO5S
Numéro CAS:
27072-45-3
Poids moléculaire :
389.38
Numéro Beilstein :
1407295
Numéro CE :
248-207-7
Numéro MDL:
MFCD00005063
Code UNSPSC :
12171500
ID de substance PubChem :
329757273
Nomenclature NACRES :
NA.32

Niveau de qualité

100

Gamme de produits

BioReagent

Pureté

≥90% (HPLC)

Fluorescence

λex 492 nm; λem 518 nm in 0.1 M phosphate pH 8.0

Adéquation

suitable for fluorescence

Température de stockage

−20°C

Chaîne SMILES 

Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1

InChI

1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H

Clé InChI

MHMNJMPURVTYEJ-UHFFFAOYSA-N

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Catégories apparentées

Description générale

Fluorescein 5(6)-isothiocyanate, also known as FITC, is a weakly acidic hydroxyxanthene whose overall size is small, with both carboxylic acid and isothiocyanate substituents on the pendant phenyl ring. The dye has a moderately sized, nonplanar aromatic system. The isothiocyanate group reacts with nucleophilic substituents (e.g. amino, hydroxyl, thiol) of biomolecules to be attached as a fluorescent label. The maximum emission of the dye is at 519nm in the same solvent.

Application

Fluorescein 5(6)-isothiocyanate (FITC) labelled molecules are used in non-microscopic immunoassays and physiological tracing experiments. FITC is widely used in flow cytometry experiments for staining proteins, nuclei, and subnuclear particles. White blood cells (WBCs) are marked with FITC to study their response toward a chemoattractant, eventually being measured.
For fluorescent labeling of proteins (e.g. immunofluorescence); H Protein labeling for gel chromatography

Conditionnement

Bottomless glass bottle. Contents are inside inserted fused cone.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Équipement de protection individuelle

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Eddie A James et al.
Arthritis & rheumatology (Hoboken, N.J.), 66(7), 1712-1722 (2014-03-26)
Rheumatoid arthritis (RA) is thought to be a T cell-mediated disease, based on its strong association with HLA class II alleles, clinical responsiveness to T cell-directed therapies, and the presence of CD4+ T cells in rheumatoid joints. The presence of
Wenhuo Hu et al.
Blood, 125(14), 2206-2216 (2015-01-31)
Hematopoietic stem cells (HSCs) possess the ability to generate all hematopoietic cell types and to self-renew over long periods, but the mechanisms that regulate their unique properties are incompletely understood. Herein, we show that homozygous deletion of the miR-29a/b-1 bicistron
Marina Vivarelli et al.
Kidney international, 87(3), 602-609 (2015-01-08)
Alloimmune antenatal membranous nephropathy (MN) during pregnancy results from antibodies produced by a neutral endopeptidase (NEP)-deficient mother. Here we report two recent cases that provide clues to the severity of renal disease. Mothers of the two children had circulating antibodies
Luisa M Villar et al.
Annals of neurology, 76(2), 231-240 (2014-06-10)
To identify a biomarker distinguishing patients who, despite a primary progressive multiple sclerosis (PPMS) clinical course, may nonetheless benefit from immune therapy. The presence or absence of both immunoglobulin (Ig) G and IgM oligoclonal bands (OCB) was blindly examined in
Benoit Malleret et al.
Blood, 125(8), 1314-1324 (2014-11-22)
Plasmodium vivax merozoites only invade reticulocytes, a minor though heterogeneous population of red blood cell precursors that can be graded by levels of transferrin receptor (CD71) expression. The development of a protocol that allows sorting reticulocytes into defined developmental stages
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