35848

2′,7′-Dichlorofluorescein

BioReagent, suitable for fluorescence, ≥90% (T)

• Synonyme(s) : DCF, Dichlorofluorescein
• Formule empirique (notation de Hill): C₂₀H₁₀Cl₂O₅
• Numéro CAS: 76-54-0
• Poids moléculaire : 401.20
• Beilstein: 58009
• Numéro CE : 200-968-6
• Numéro MDL: MFCD00005047
• Code UNSPSC : 12171500
• ID de substance PubChem : 57649439
• Nomenclature NACRES : NA.32

Propriétés

• Gamme de produits: BioReagent
• Niveau de qualité: 100
• Essai: ≥90% (T)
• Forme: solid
• Pf: 280 °C (dec.) (lit.)
• Fluorescence: λ_ex 504 nm; λ_em 529 nm in 0.1 M Tris pH 8.0
• Adéquation: suitable for fluorescence
• Chaîne SMILES: Oc1cc2Oc3cc(O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl
• InChI: 1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H
• Clé InChI: VFNKZQNIXUFLBC-UHFFFAOYSA-N

Description

Description générale

2′,7′-Dichlorofluorescein, also known as DCF is a fluorescent dye. The DCF fluorescence excitation is at 498nm with emission at 522nm. The lipophilic non-fluorescent Dcfh-DA readily crosses the cell membrane through passive diffusion followed by deacetylation. The deacylated product is an oxidant sensitive 2′,7′-dichlorofluorescein (DCHF). DCHF is oxidized later to form highly fluorescent DCF, which is measured.

Application

2′,7′-Dichlorofluorescein is used as fluorescent probe to measure ROS (Reactive Oxygen Species) for biological studies. It may also be used as a dip reagent for the fluorometric determination of vicinal diols, glycosides, and phenols.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Gloves