18507
Atto 565 maleimide
BioReagent, suitable for fluorescence
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About This Item
Formule empirique (notation de Hill):
C37H37ClN4O10
Poids moléculaire :
733.16
Numéro MDL:
Code UNSPSC :
12352111
Nomenclature NACRES :
NA.32
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Gamme de produits
BioReagent
Niveau de qualité
Essai
>90.0% (HPLC)
Forme
powder
Fabricant/nom de marque
ATTO-TEC GmbH
Transmittance
254 nm
565 nm
Fluorescence
λex 563 nm; λem 592 nm in 0.1 M phosphate pH 7.0
λ
(ethanol with 0.1% trifluoroacetic acid)
Absorption UV
λ: 560-566 nm Amax
Adéquation
corresponds for coupling to thiols
suitable for fluorescence
Température de stockage
−20°C
Application
Atto 565 is a new label with high molecular absorption (120.000) and quantum yield (0.9) as well as sufficient stoke′s shift (excitation maximum 563 nm, emission maximum 592 nm). Due to an insignificant triplet formation rate it is well suited for single molecule detection applications. Maleimides are well suited for coupling to thiol groups. This is similar to iodacetamides, but maleimides do react more thiol selective. They do not show significant reaction with histidine or methionine. Hydrolysis of maleimides to a mixture of isomeric nonreactive maleamic acids can compete significantly with thiol modification, particularly above pH 8. Maleimides may be used for labeling of amines, which usually requires a higher pH than reaction of maleimides with thiols.
Informations légales
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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