10831
Histopaque®-1083
sterile-filtered, density: 1.083
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About This Item
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75
Produits recommandés
Stérilité
sterile-filtered
Niveau de qualité
Forme
liquid
Durée de conservation
Expiry date on the label.
Technique(s)
cell culture | mammalian: suitable
Impuretés
endotoxin, tested
Densité
1.083
Application(s)
hematology
histology
Température de stockage
2-8°C
Description générale
Histopaque®-1083 is a solution containing polysucrose and sodium diatrizoate, adjusted to a relative density of 1.083 g/mL. It facilitates the recovery of viable mononuclear cells from small volumes of blood of rats, mice, and other small mammals.
Application
Histopaque®-1083 has been used for the isolation of mononuclear cells and leukocytes from rat peripheral blood.
Caractéristiques et avantages
Histopaque®-1083 offers numerous advantages, such as:
- Optimal recovery of viable cells
- Permits selective separation of blood cell types
- Prevents cell distortion
- Preserves cell viability
- Minimal extraneous cell interference
- Reproducible performance from batch to batch
- Sterile-filtered and endotoxin-tested
- Stable at 2-8°C for at least 2 years when protected from light
Principe
Anticoagulated blood is layered onto HISTOPAQUE1083. During centrifugation, erythrocytes are aggregated by polysucrose and rapidly sediment; whereas, lymphocytes and other mononuclear cells remain at the plasma-HISTOPAQUE 1083 interface. Erythrocytes pellet to the bottom of the centrifuge tube.
Informations légales
Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
Code de la classe de stockage
8A - Combustible corrosive hazardous materials
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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