RDD039

Imidazole

for molecular biology, ≥99% (titration), free-flowing, Redi-Dri^™

• Synonyme(s) : 1,3-Diaza-2,4-cyclopentadiène, Glyoxaline
• Formule empirique (notation de Hill): C₃H₄N₂
• Numéro CAS: 288-32-4
• Poids moléculaire : 68.08
• Numéro Beilstein : 103853
• Numéro MDL: MFCD00005183
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.21

Propriétés

• Qualité: for molecular biology; free-flowing
• Niveau de qualité: 100
• Pression de vapeur: <1 mmHg ( 20 °C)
• Gamme de produits: Redi-Dri^™
• Pureté: ≥99% (titration)
• Forme: crystalline powder
• pH: 6.2-7.8 (25 °C)
• pKa (25 °C): 6.95
• Point d'ébullition: 256 °C (lit.)
• Pf: 88-91 °C (lit.)
• Application(s): diagnostic assay manufacturing
• Activité étrangère: DNase, RNase and protease, none detected
• Chaîne SMILES: c1c[nH]cn1
• InChI: 1S/C3H4N2/c1-2-5-3-4-1/h1-3H,(H,4,5)
• Clé InChI: RAXXELZNTBOGNW-UHFFFAOYSA-N

Description

Description générale

Imidazole is a heterocyclic compound with a five-membered planar ring. It is amphoteric and highly polar.

Application

Imidazole has been used

• in the lysis, wash, and elution buffer for the purification of histidine tagged proteins
• in gradient for the purification of histidine tagged proteins using nickel affinity chromatography
• as a component of homogenization buffer for the purification of phagosomal compartments from dendritic cells

Excellent pour les tampons compris dans la plage de pH 6,2-7,8

Actions biochimiques/physiologiques

Imidazole derivatives have antibacterial, antifungal and anticancer functionality. It interacts with DNA and also binds to protein and stops cell division. It also acts as a microtubule destabilizing agents and inhibits topoisomerase and Cytochrome P450 Family 26 Subfamily A Member 1 (CYP26A1) enzymes. Imidazole based anticancer drug find applications in cancer chemotherapy. It is used as buffer component for purification of the histidine tagged recombinant proteins in immobilized metal-affinity chromatography (IMAC).

Caractéristiques et avantages

• Qualified for molecular biology applications, DNase, Rnase, and protease free
• Redi-Dri^™ packaging controls moisture, eliminating clumps
• Crystalline powder remains free-flowing and much easier to use
• Safer to handle and weigh out for routine use in lab
• Less waste, loss of drying, while maintaining high quality

Autres remarques

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Informations légales

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Informations sur la sécurité

• Pictogrammes: GHS08,GHS05,GHS07
• Mention d'avertissement: Danger
• Mentions de danger: H302,H314,H360D
• Conseils de prudence: P260 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Classification des risques: Acute Tox. 4 Oral - Eye Dam. 1 - Repr. 1B - Skin Corr. 1C
• Code de la classe de stockage: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): 293.0 °F - closed cup
• Point d'éclair (°C): 145 °C - closed cup