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38266

Millipore

Ferrioxamine E from Streptomyces antibioticus

suitable for microbiology, ≥95%

Synonyme(s) :

1,12,23-Trihydroxy-1,6,12,17,23,28-hexaazacyclotritriacontane-2,5,13,16,24,27-hexone Iron(III) complex

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About This Item

Formule empirique (notation de Hill):
C27H45FeN6O9
Numéro CAS:
Poids moléculaire :
653.53
Numéro MDL:
Code UNSPSC :
41171614
ID de substance PubChem :
Nomenclature NACRES :
NA.85

Stérilité

non-sterile

Niveau de qualité

Pureté

≥95% (HPLC)
≥95%

Forme

powder

Perte

≤6% loss on drying

Application(s)

environmental
food and beverages

microbiology

Température de stockage

2-8°C

Adéquation

Salmonella spp.

Chaîne SMILES 

[Fe+3].[O-]N1CCCCCNC(=O)CCC(=O)N([O-])CCCCCNC(=O)CCC(=O)N([O-])CCCCCNC(=O)CCC1=O

InChI

1S/C27H45N6O9.Fe/c34-22-10-14-26(38)32(41)20-8-3-6-18-30-24(36)12-15-27(39)33(42)21-9-2-5-17-29-23(35)11-13-25(37)31(40)19-7-1-4-16-28-22;/h1-21H2,(H,28,34)(H,29,35)(H,30,36);/q-3;+3

Clé InChI

MZFKJKOHYACYNT-UHFFFAOYSA-N

Description générale

Scientifically proven supplement for culture media improves detection of Salmonella, Enterobacter sakazakii and Yersinia enterocolitica .

Ferrioxamine E from Streptomyces antibioticum is a siderophore which facilitates the supply of iron (III), an essential trace element, to bacteria involved in food poisoning, including Salmonella, Enterobacter saka-zakii and Yersinia enterocolitica. It promotes rapid growth by reducing the lag phase in culture media and reactivates dormant bacteria. Use of ferrioxamine E can permit rapid detection of low bacterial cell counts, even from dry products like spices and tea after long storage periods. It is an essential component of quality control efforts in the food industry.

Ferrioxamine E is often used in Buffered Peptone Water, the medium recommended by the ISO-Norms for Enterobacteriacea. Ferrioxamine E also improves the motility of Salmonella, which helps to improve the identification by semisolid selective motility media like SIM, MRSV, DIASSALM or SMS. Ferrioxamine E does not improve growth of Escherichia coli, Shigella, Proteus, Providencia and Morganella species; a feature that makes it a semi-selective compound. The recommended concentration of Ferrioxamine E to promote bacterial growth is between 5 and 200 ng/mL
The ferrioxamines are basically metabolic products produced by the actinomycetes, and are characterised as ferrioxamines A, B, C, D 1, D2, E, F and G. Generally all ferrioxamines are trihydroxamates and the deferrioxamines are either linear or cyclic (D2 and E).

Application

Supplementation of pre-enrichment broth and enrichment broth media for improved recovery of Salmonella from artificially or naturally contaminated foods

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


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Consulter la Bibliothèque de documents

The crystal structure of ferrioxamine E
Helm VD
Journal of the American Chemical Society, 98 (1) (1976)
Milos Petrik et al.
Nuclear medicine and biology, 39(3), 361-369 (2011-12-17)
Siderophores are low-molecular-mass iron chelators serving as iron transporters for almost all bacteria, fungi and some plants. Iron is an essential element for majority of organisms and plays an important role in virulence of pathogenic organisms. (68)Ga is a positron
R Reissbrodt et al.
Zentralblatt fur Bakteriologie : international journal of medical microbiology, 279(3), 344-353 (1993-08-01)
Salmonella can overcome the iron limitation of iron-limited nutrient media and albumen by utilizing the siderophores ferrioxamine E and G und grow. 14 out of 18 food-poisoning Salmonella strains of different serotypes stored in hen's egg albumen (approx. 2 organisms/ml)
R Reissbrodt et al.
Applied and environmental microbiology, 68(10), 4788-4794 (2002-09-27)
Salmonella enterica serovar Typhimurium and enterohemorrhagic Escherichia coli were stressed by prolonged incubation in water microcosms until it was no longer possible to observe colony formation when samples were plated on nonselective medium. Overnight incubation of samples in nutrient-rich broth
H Heinonen-Tanski et al.
Microbiological research, 155(1), 65-68 (2000-06-01)
The effect of a siderophoric compound, ferrioxamine E, in the pre-enrichment broth on determining of Salmonella infantis in environmental samples was tested with combination of various pre-enrichment times and enrichment temperatures of 37 and 43 degrees C. Ferrioxamine E slightly

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