ROAHA

Anti-HA (12CA5)

from mouse IgG_2bκ

• Synonyme(s) : antibody
• Code UNSPSC : 12352203

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Conjugué: unconjugated
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 12CA5, monoclonal
• Pureté: >90% (HPLC and SDS-PAGE)
• Forme: frozen liquid (11666606001); lyophilized (11583816001)
• Conditionnement: pkg of 200 μg (11583816001); pkg of 5 mg (11666606001 [1 ml])
• Fabricant/nom de marque: Roche
• Concentration: 5 mg/ml (11666606001)
• Isotype: IgG2bκ
• Séquence de l'épitope: YPYDVPDYA
• Température de stockage: −20°C

Description

Description générale

Hemagglutinin HA is a dominant antigen present on the influenza viral surface. HA is a homotrimer, in which each monomer is produced as a single polypeptide. This monomer is cleaved into two subunits HA1 and HA2 by the host protease. The HA gene is mapped to segment 4 of influenza B viral genome.

Spécificité

Anti-HA (12CA5) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal).

Immunogène

Amino acids 98-106 from the human influenza virus hemagglutinin protein.

Application

Use Anti-HA (12CA5) for the detection of native influenza hemagglutinin protein and recombinant HA-tagged proteins using:

• Dot blots
• Immunochemistry
• Immunoprecipitation
• Western blotting

Note: For experiments in which sensitivity is not critical, use Anti-HA (12CA5). For higher sensitivity detection in western blotting at 10-fold lower concentration, use Anti-HA High Affinity (3F10).

Actions biochimiques/physiologiques

Membrane fusion and receptor-binding action is the key function of hemagglutinin (HA) and it aids in viral entry and infection. HA is an influenza-virus glycoprotein that regulates membrane fusion and receptor-binding functions during viral entry and infection. The virus gains entry into the host cell via endocytosis and successive membrane fusion mediated by the HA antigen. HA plays a crucial role in viral pathogenesis and host response to viral infection.

Qualité

Each lot of Anti-HA antibody is tested for immunoreactivity and purity relative to a reference standard.

Notes préparatoires

Working concentration: Working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:

• Dot blot: 0.1 to 1 μg/ml
• Immunfluorescence: 1-10 μg/ml
• Western blot: 0.1 to 1 μg/ml

Reconstitution

11 583 816 001: Add 0.5 ml of PBS to a final concentration of 0.4 mg/ml.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Informations sur la sécurité

• Code de la classe de stockage: 13 - Non Combustible Solids
• Point d'éclair (°F): does not flash
• Point d'éclair (°C): does not flash