RDLIG-RO
Roche
Rapid DNA Dephos & Ligation Kit
sufficient for 40 reactions (04898117001), sufficient for 160 reactions (04898125001), optimum reaction temp. 37 °C
Synonyme(s) :
cloning of dna fragments, dna ligase, ligases
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About This Item
Produits recommandés
Utilisation
sufficient for 160 reactions (04898125001)
sufficient for 40 reactions (04898117001)
Niveau de qualité
Fabricant/nom de marque
Roche
Paramètres
37 °C optimum reaction temp.
Température de stockage
−20°C
Description générale
The Rapid DNA Dephos & Ligation Kit enables fast and efficient dephosphorylation and ligation of sticky- or blunt-end DNA fragments.
Spécificité
Specificity: rAPid Alkaline Phosphatase
Alkaline Phosphatase catalyzes the hydrolysis of numerous phosphate esters, such as esters of primary and secondary alcohols, saccharides, cyclic alcohols, phenols and amines. Phosphodiesters do not react. The enzyme hydrolyzes inorganic pyrophosphate. The kinetic properties of the enzyme depend on many factors, such as purity of enzyme, concentration of enzyme in the assay, buffer, pH etc.
Heat inactivation: rApid Alkaline Phosphatase:
Two minutes at 75 °C.
T4 DNA Ligase:
10 minutes at 65 °C.
Alkaline Phosphatase catalyzes the hydrolysis of numerous phosphate esters, such as esters of primary and secondary alcohols, saccharides, cyclic alcohols, phenols and amines. Phosphodiesters do not react. The enzyme hydrolyzes inorganic pyrophosphate. The kinetic properties of the enzyme depend on many factors, such as purity of enzyme, concentration of enzyme in the assay, buffer, pH etc.
Heat inactivation: rApid Alkaline Phosphatase:
Two minutes at 75 °C.
T4 DNA Ligase:
10 minutes at 65 °C.
Application
The Rapid DNA Dephos & Ligation Kit minimizes the time needed to perform diverse applications, including:
- Cloning of fragments into either plasmid or phage vectors
- Linker ligation
- Recircularization of linear vector DNA
- Generation of libraries
Conditionnement
1 kit containing 5 components
Définition de l'unité
Unit Definition: rAPid Alkaline Phosphatase
One unit of rAPid Alkaline Phosphatase is the enzyme activity which hydrolyzes one μmol of 4-nitrophenyl phosphate in one minute at +37 °C under assay conditions.
Volume Activity: rApid Alkaline Phosphatase: 1 U/μl
T4 DNA Ligase: 5 U/μl
One unit of rAPid Alkaline Phosphatase is the enzyme activity which hydrolyzes one μmol of 4-nitrophenyl phosphate in one minute at +37 °C under assay conditions.
Volume Activity: rApid Alkaline Phosphatase: 1 U/μl
T4 DNA Ligase: 5 U/μl
Autres remarques
For life science research only. Not for use in diagnostic procedures.
Composants de kit seuls
Réf. du produit
Description
- rAPid Alkaline Phosphatase Buffer 10x concentrated
- rAPid Alkaline Phosphatase 1 U/μl
- DNA Dilution Buffer 5x concentrated
- T4 DNA Ligation Buffer 2x concentrated
- T4 DNA Ligase 5 U/μl
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
does not flash
Point d'éclair (°C)
does not flash
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