11582950001

BM Chemiluminescence ELISA Substrate (POD)

• Synonyme(s) : ELISA substrate
• Code UNSPSC : 12352204

Propriétés

• Forme: solution
• Niveau de qualité: 100
• Conditionnement: pkg of 250 mL (for 2500 wells or 1000 tubes)
• Fabricant/nom de marque: Roche
• Conditions de stockage: (Keep container tightly closed in a dry and well-ventilated place.)
• Technique(s): ELISA: suitable
• Couleur: clear colorless
• Solubilité: water: miscible
• Adéquation: suitable for chemiluminescence
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C

Description

Description générale

The sensitivity of enzyme-linked immunosorbent assays often depends on the detection limit of the colorimetric substrates used. In addition, the dynamic range of a colorimetric assay is restricted to a maximum of two orders of magnitude due to the physical/chemical limitations of absorbance measurement (Law of Lambert & Beer). With chemiluminescence technology, a nonradioactive method, which combines the convenience of tube or microplate-based immunoassays with the advantages of isotopic assays, is now available. The BM Chemiluminescence ELISA Substrate (POD) has been evaluated with different immunoassays on the LB 96 P microplate reader and the LB 953 tube chemiluminescence analyzer from EG&G;Berthold.

Application

The BM Chemiluminescence ELISA Substrate (POD) provides a substrate solution for peroxidase-based (POD, HRP) secondary detection systems with highly sensitive, enhanced chemiluminescence. This reagent has been optimized for ELISA applications to be run on a microplate chemiluminescence reader (96-well format) or tube-format luminometers.

Caractéristiques et avantages

• Improved sensitivity
• Large dynamic range
• Rapid and constant signal

Contents
Substrate Reagent A (buffered solution that contains luminol/4-iodophenol)
Starting Reagent B (buffered solution that contains a stabilized form of H₂O₂)

Principe

Horseradish peroxidase (POD, HRP), in the presence of hydrogen peroxide (H₂O₂), catalyzes the oxidation of diacylhydrazides such as luminol. A reaction product in an excited state is thus formed, then decays to the ground state by emitting light. Strong enhancement of the light emission is achieved by the agent 4-iodophenol (contained), which acts as a radical transmitter between the formed oxygen radical and luminol.

Notes préparatoires

Working solution: Preparation of working solution

The BM Chemiluminescence ELISA Substrate is supplied as a set of two stable solutions. Depending on the scale of the assay, the appropriate amount of substrate solution has to be prepared 15 minutes before use:

• Add to 100 parts of solution A one part of solution B.
• Stir the mixture for at least 15 minutes at 15 to 25 °C to equilibrate the components.

Premixed working solution stored at 2 to 8 °C should be warmed up to 15 to 25 °C to avoid temperature effects during measurement.
Storage conditions (working solution): The premixed solution is stable for 1 week, when stored at 2 to 8 °C.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): does not flash
• Point d'éclair (°C): does not flash