10223565001
Roche
Histone
from calf thymus
Synonyme(s) :
histone
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About This Item
Code UNSPSC :
12352204
Produits recommandés
Niveau de qualité
Forme
lyophilized
Conditionnement
pkg of 10 mg
Fabricant/nom de marque
Roche
Conditions d'expédition
wet ice
Température de stockage
2-8°C
Catégories apparentées
Description générale
Mixture of histones H1, H2A, H2B, H3, and H4, isolated from calf thymus.
Histones are a group of DNA-binding proteins that are characterized by relatively high levels of lysine and arginine. Five different fractions of histones have been isolated and characterized. These are named as H1, H2A, H2B, H3 and H4. The H1 fraction is lysine-rich, while the H2A and H2B are slightly lysine-rich, The H3 and H4 fractions are arginine-rich. The molecular weights of histones are approximately 11 to 21 kDa depending on the fraction.
Histones are a group of DNA-binding proteins that are characterized by relatively high levels of lysine and arginine. Five different fractions of histones have been isolated and characterized. These are named as H1, H2A, H2B, H3 and H4. The H1 fraction is lysine-rich, while the H2A and H2B are slightly lysine-rich, The H3 and H4 fractions are arginine-rich. The molecular weights of histones are approximately 11 to 21 kDa depending on the fraction.
Application
Histone from calf thymus has been used:
- in in vitro kinase assay to check the phosphorylation of histones by protein kinase A.
- to check the in vitro methyltransferase activity of protein argininemethyltransferases from Oryza sativa (OsPRMTs).
- in anti-histone ELISA.
Actions biochimiques/physiologiques
Histones have an important role in the organization and modification of chromatin. The nucleosome is the basic unit of the chromatin. It is made up of two molecules each of histones H2A, H2B, H3 and H4, wrapped with approximately 146bp of DNA. Histone H1 binds to the nucleosomes at the DNA entry and exit points and stabilizes them.
Forme physique
Lyophilizate (natural mixture of histones H1, H2A, H2B, H3, and H4).
Notes préparatoires
Working concentration: 1 to 5 mg/ml.
Working solution: Recommended solvent is double-distilled water.
Storage conditions (working solution): Aqueous solution is stable at -15 to -25 °C for several months.
Avoid repeated freezing and thawing!
Working solution: Recommended solvent is double-distilled water.
Storage conditions (working solution): Aqueous solution is stable at -15 to -25 °C for several months.
Avoid repeated freezing and thawing!
Autres remarques
For life science research only. Not for use in diagnostic procedures.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Les clients ont également consulté
Nicole Happel et al.
Gene, 431(1-2), 1-12 (2008-12-09)
The lysine-rich H1 histone family in mammals includes eleven different subtypes, and thus it is the most divergent class of histone proteins. The central globular H1 domain asymmetrically interacts with DNA at the exit or entry end of the nucleosomal
Evgeny A Ermakov et al.
International journal of molecular sciences, 21(19) (2020-10-04)
Schizophrenia is known to be accompanied not only with an imbalance in the neurotransmitter systems but also with immune system dysregulation and chronic low-grade inflammation. Extracellular histones and nucleosomes as damage-associated molecular patterns (DAMPs) trigger systemic inflammatory and toxic reactions
Ona Bloom et al.
Proceedings of the National Academy of Sciences of the United States of America, 108(25), 10255-10259 (2011-06-08)
Systemic lupus erythematosus (SLE) is a chronic autoimmune disease characterized by the presence of pathogenic autoantibodies, many of which are directed against nuclear antigens, in particular double-stranded (ds) DNA. Both clinical studies and animal models have shown that anti-dsDNA antibodies
H1 histone and the condensation of chromatin and DNA.
R D Cole et al.
Cold Spring Harbor symposia on quantitative biology, 42 Pt 1, 253-263 (1978-01-01)
Core histones H2B and H4 are mobilized during infection with herpes simplex virus 1.
Conn KL, et.al
Journal of Virology, 85(24), 13234-13252 (2011)
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